Dear Cynthia,
I would be very interested in your mouse brain processing protocol, 
also.  We have been having some terrible problems with wrinkling and 
"shredding".  At first we thought this could be overdehydration or 
even fixation.  We have tried three different processing schedules 
and also changed the fixation time.  Still having problems, but 
sectioning is a little better.  Would like to compare other's 
schedules to ours.
Any other responses would also be welcome.
Thanks,
Jo Dee Fish

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Jo Dee Fish
Research Technologist II
Microscopy Core
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 695-3720
Fax: (415) 285-5632
E-mail: jfish@gladstone.ucsf.edu

Mailing address:
Gladstone Institutes
P.O. Box 419100
San Francisco, CA 94141-9100
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