I'm using the patient tissue as a negative control, not applying the primary
antibody.  We use the Dako autostainer and have not had problems with any
other antibodies.  The first time this happened, the sample was a cell block
of a pleural fluid, the second time a lung biopsy.

> ----------
> From: 	Carrie Kyle-Byrne[SMTP:ckbyrne@exelixis.com]
> Sent: 	Tuesday, March 26, 2002 3:34 PM
> To: 	George, Cheryl
> Cc: 	'HistoNet@Pathology.swmed.edu'
> Subject: 	Re: Calretinin
> 
> what are you using for your negative control and how are you doing your
> staining (manual or automated...which machine)?  what type of tissue is
> the
> patient specimen?
> carrie
> 
> ----- Original Message -----
> From: "George, Cheryl" 
> To: 
> Sent: Tuesday, March 26, 2002 10:57 AM
> Subject: Calretinin
> 
> 
> > Hi out there,
> >    We do calretinin on human FFPE tissue and over the last couple of
> weeks
> > have had two cases where the patient negative control tissue stains just
> as
> > brightly and specifically as the positive.  Other markers on the same
> > patient tissue don't react this way and I was wondering if anyone has an
> > idea about what could cause this.
> >
> > Thanks in advance!!
> >
> > Cheryl
> >
> > Cheryl George, BS, HT (ASCP)
> > Anatomic Pathology Supervisor
> > Elliot Hospital Laboratory
> > One Elliot Way
> > Manchester, NH 03103
> > (603) 663-2686
> >
> >
> >
> >
> >
>