I'm using the patient tissue as a negative control, not applying the primary
antibody. We use the Dako autostainer and have not had problems with any
other antibodies. The first time this happened, the sample was a cell block
of a pleural fluid, the second time a lung biopsy.
> From: Carrie Kyle-Byrne[SMTP:firstname.lastname@example.org]
> Sent: Tuesday, March 26, 2002 3:34 PM
> To: George, Cheryl
> Cc: 'HistoNet@Pathology.swmed.edu'
> Subject: Re: Calretinin
> what are you using for your negative control and how are you doing your
> staining (manual or automated...which machine)? what type of tissue is
> patient specimen?
> ----- Original Message -----
> From: "George, Cheryl"
> Sent: Tuesday, March 26, 2002 10:57 AM
> Subject: Calretinin
> > Hi out there,
> > We do calretinin on human FFPE tissue and over the last couple of
> > have had two cases where the patient negative control tissue stains just
> > brightly and specifically as the positive. Other markers on the same
> > patient tissue don't react this way and I was wondering if anyone has an
> > idea about what could cause this.
> > Thanks in advance!!
> > Cheryl
> > Cheryl George, BS, HT (ASCP)
> > Anatomic Pathology Supervisor
> > Elliot Hospital Laboratory
> > One Elliot Way
> > Manchester, NH 03103
> > (603) 663-2686
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