Hi HistoNetters

Patsy Ruegg pointed out that formic acid has proven to be a good agent for
decalcification with subsequent IHC.  I don't disagree.  In fact, more of my
customers use some form of formic acid decalcifier than anything else.  I
have just found, in my own empirical experience, that I have gotten superior
results using EDTA.

Patsy also asked if microwaving itself affects the results, and the answer
is that it only speeds them up.  Decalcification speeds can be highly
temperature dependent.

Furthermore, the use of an effective stirrer can ensure that fresh
decalcification fluid is always in contact with the specimen.

best regards,
Steven Slap

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Marketing Manager/Microwave Product Specialist
Hacker Instruments & Industries, Inc.
http://www.hackerinstruments.com
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