Dear Histonetters,

I quit using PBS w ith tween 20 approximately 4 years ago. I have had
great success with using just DI H2O as my rinse. If I am going to store
my slides for a while before I can continue with my staining, then I use
PBS for a holding solution.
 
I am talking about FFPE tissue of course.

Sorry if I am alittle late responding, I was away on vacation last week.

Marsha Price

On Thu, 21 Feb 2002 17:56:47 -0500 Amos Brooks 
writes:
> Thanks,
>     Part of the senseless babbling I was referring to involves 
> osmotic
> balance but it didn't make much sense the way I described it. I'm 
> glad that
> I was on the same wavelength though.
>     The tissue is formalin fixed and paraffin embedded. So does that 
> prevent
> the deleterious effects? If it does then people are wasting a lot of 
> money
> on buffer. I for one am not quite ready to take such a chance but it 
> bears
> consideration.
> Amos
> 
> ----- Original Message -----
> From: "Monson, Frederick C." 
> To: "'Amos Brooks'" 
> Cc: "'List-HistoPath'" 
> Sent: Thursday, February 21, 2002 4:26 PM
> Subject: RE: Spin on IHC buffers
> 
> 
> > Hi Amos,
> > One of my favorite sub-subjects.  Colligative properties of
> > solutions.  I think the words you want are "osmotic pressure".  If
> sections
> > are fresh-frozen and thick enough to house unsectioned cells, then 
> just as
> > in a suspension of RBC's, diHOH will 'fill them to bursting', 
> because of
> > "osmosis".   Same thing to the unfixed "ground substance" of 
> connective
> > tissue.  Maybe I should issue forth on "Chromatin" and 
> "Metachromasia".
> Ah
> > yes!  My alcohol of choice for dehydrating tissues stained with
> > metachromatic dyes is Tertiary ButAnal ("TBA" - Now why would 
> anyone think
> > to  give the suffix of that word so much prominence?)  In any 
> case, with
> > TBA, the metachromasia assuredly survives.  I've had some in Azure 
> B (DNA
> > vs. RNA) mounted in Damar for 20 years and still see azure and 
> purple.
> > Get those people free tickets to see "Osmosis Jones".
> > By the way, if the tissue is fixed, diHOH probably won't have any
> > deleterious effect on the results.  I mean, after we use the magic 
> tap
> water
> > on the Schiff's Reagent, don't we rinse in diHOH before we enter 
> the
> > alcohols.  No?  For Heaven's sake, why not!!!???!!!  Wouldn't we
> contaminate
> > the alcohol dilutions we made with diHOH?
> > Has anyone ever determined why the tap water in Duluth works 
> better
> > on Schiff's than that in Minneapolis?  I never did hear how that 
> debate
> was
> > resolved.  I missed most of it by becoming electron microscopic.
> > Well, Amos, I am taking tomorrow off, because I need to replace a
> > windshield wiper motor.  I'm starting at the exhaust pipe and I'm 
> going to
> > work my way forward until I find that little bugger.  Then, after 
> lunch,
> > while I revel in my mechanical skills, I'm going to watch the US 
> team beat
> > the Russians in ice hockey and, hopefully, the Canadians beat the
> > Liliputians.
> >
> > My best wishes for all to have a peaceful weekend, especially our
> > neighbors who are sailing with the Navy, flying with the Air Force
> > (robotically with the Army) and sand flea hunting with the 
> Marines.
> Smack!
> >
> > Fred Monson
> >
> > Frederick C. Monson, PhD
> > The best research
> > Center for Advanced Scientific Imaging
> > occurs before work
> > West Chester University
> > at the bench.
> > West Chester, Pennsylvania, USA, 19383
> > 610-738-0437
> > fmonson@wcupa.edu
> 
> 

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