vimentin control for fixation in IHC

From:C & J Haley <haley@primary.net>

We use the Vimentin (V9 clone)  from Biogenex.   I would like to expand
this discussion.  I have never felt the vimentin was the best indicator
of immunoreactivity.  Maybe if you were only working with intermediate
filaments it would be okay.  We all know that Ki67, ER, PR, Her2, and
about any CD marker would not survive the abuse that Vimentin does.  So
what are the opinions on Vimentin's relevance to tissue
immunoreactivity?

Just curious,

Jane Haley




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