vimentin control for fixation for immunos -Reply

From:Tony Henwood <>

The usual clone for IPX control is V9. The other clone I have used is 3B4.
This requires HIER for success, so as a test for fixation and processing I
would think its use to be limited. It is interesting to note the dramatic
difference in staining after using HIER prior to V9 IPXs. Lymphocyte
marking is especially enhanced.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> "Hagerty, Marjorie A." <> 7/March/2001 12:19pm
When you run a vimentin on your patient slide to check fixation, do you
a specific clone? I heard quite awhile ago that it was a specific clone.
Those of you using vimentin as a test for immunoreactivity, what clone
you use? I assume (?) that you treat this slide identically to the other
slides on the case as far as antigen retrieval.

Thanks in advance,

<< Previous Message | Next Message >>