RE: Antigen Retrieval Solution, Citrate Buffer

Citrate buffer for antigen retrieval:

Citric acid 21g
distilled water 10 litres

To this add 2N sodium hydroxide solution (12g in 150 ml of distilled water) until pH 6.8
most of the 150ml will be used.

This solution keeps for weeks.

We use it for both microwave and pressure cooking.  Make sure the pressure cooker is not aluminum!

Steve Machin
Chief Medical Laboratory Scientific Officer
Histopathology and Cytology
Bassetlaw District General Hospital
Kilton Hill
S81 0BD

Tel UK 01909 500990 Exnt 2492
Fax UK 01909 502480

8am to 4:15pm UK time

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