Re: PFK's

From:Neuropathology <>

Mount in an aqueous mountant after killing the reaction in formalin.  Forget
the acetone, alcohols and xylene.

Andy Shand

----- Original Message -----
From: Klassen, Shannon SDH <>
To: 'Histonet' <>
Sent: Thursday, March 01, 2001 7:23 PM

> Help  I need a method for phosphofructokinase for frozen muscle tissue -
> method isn't working well and the reference is very old and I don't have
> access to it.
> Or  is there a enzyme genius out there who can help me?
> Stain done on freshly cut frozen sections - no fixation - go
> straight into incubation media for 1 hour @ 37C.  Rinse thru 30% and 60%
> acetone and then distilled water.  Everything appears good up to this
> Then dehydrate thru alcohols and xylene - this is where the reaction fades
> to almost nothing.  Should I just leave them in water and mount with an
> aqueous mount??????  I don't really want to play around with the stain too
> much as the chemicals can be a little pricey.
> Thanks for the help in advance.  Shannon

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