tyramide amplification TSA vs CSA

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
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The TSA kit is available from New England Nuclear, NEN and is the same
technology as the CSA kit (which may have some buffer or other variations).

Du Pont aka NEN sold the technology to DAKO, so they will be basically the

You need to go back in the Histonet Archives, there was a load of
discussion on this some time ago, and some people preferred the TSA kit to
the CSA kit, but washing was critical, plus dilution of primary and
secondary antibodies.  

If you need help, you can contact Dr. Mark Bobrow at NEN who was the
originator of the technic, bobrowmn@nenlifesci.com, and a very nice fellow!
 There is tons of info in the literature, about 2 -3 years ago, in J of
Histochem Cytochem.  

Mark Bobrow will certainly steer you in the right direction and I also know
that Barb Wright has used this technology, her comments and technics are in
archives, and were very helpful.  Hopefully she will come forth and visit
with you.

Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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