Richard-Allan Stains
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From: | Linda Jenkins <jlinda@ces.clemson.edu> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Laurie Colbert wondered, "Is Richard Allan a common denominator
with everyone who is having problems?????"
My thoughts on this - I have used Richard-Allan hematoxylin, eosin,
clarifier, bluing, and cytology stains practically since their arrival in
the marketplace (late '70's - mid-eighties?). The ONLY consistent,
reliable procedure I've NEVER had to "tweak" in the last 20 years has been
my H &E stain - as it is beautiful! In fact, in 1990, when I was first
introduced to the gentleman (Jerry Fredenburg) who was responsible for the
development of these wonderful chemicals I immediately hugged him and said,
"Thank You, Thank You, Thank You!" In fact, all of our area labs have used
these stains with great results.
Now, in all fairness, I must add that I use real Xylenes for
clearing and graded ethanols for dehydration. Have not been seen
consistent results with substitutes.
And, as a final note, I remember thinking that the original
question that started this query had a processing procedure that went
straight from formalin to 100%alcohol. Isn't this a little odd? Doesn't
everyone use graded alcohols?
Oh, well...I just didn't want techs, who've never used them, to get
the wrong impression about Richard-Allan stains.
Linda
*********************************
Linda Jenkins, HT
Clemson University
Department of Bioengineering
864.656.5553
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