Fixatives for crow and Avian Tissue

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From:Gwin Kerce <>
To:histonet <>
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We are just doing light microscopy. It is not the ultrastructure that is the
trouble ( my mistake). The formalin fixation
does not have the crips detail that is desired. We are working on a study of
35 week old leghorns ( male obviously).
The calcium deposit very in size and the tissue is sectioned undecalcified.

> Our lab is working on a project that involves locating calcium deposits
> in avian testis and epididymis.  We have tried to use Bouin's Solution,
> unfortunately,
> it dissolves the deposits of interest.  We also tried 10% Buffered
> Foramlin, however it lacks the desired crispness.  Does anyone
> have any suggestions for a fixative?  We need to fix both the testicular
> and epididmyal tissue.

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