Re: citrate buffer recipe

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From:Debbie Gae Pepperall <>
To:"Sebree Linda A." <>
Content-Type:text/plain; charset="us-ascii"

At 02:42 PM 27/03/00 -0600, you wrote:
>Our lab would like to make its own citrate buffer for HIER purposes; if
>anyone out there has a "tried and true" recipe for such, we would appreciate
>hearing from you.
>Thanks in advance,
>Linda Sebree
>Hi Linda

We have 2 methods, both work great. Depending on how busy you are to make
these up....

2.1 grams citric acid in 1 litre/liter of distilled water
pH to 6.0 with 1M NaOH
Soln A : 10.5 grams citric acid in 500 ml distilled water
Soln B : 29.4 grams sodium citrate in 1 litre of distilled water
Mix 41 ml of A + 9 ml of B, make upto 500ml with distilled water, PH to 6.0
with 1M NaOH.

Have fun.

Zenobia Haffajee
HAPS, Newcastle, NSW, "Down Under".>
>Linda A. Sebree, HT
>University of Wisconsin Hospital & Clinics
>Immunohistochemistry/In Situ Hybridization Laboratory
>600 Highland Avenue
>Madison, WI  53792-2472
>FAX: (608)263-1568

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