Amos,
have you heard of Tyramide Signal Enhancement? It works great and is easy
to use.
Marsha Price

On Sun, 26 Mar 2000 09:14:54 -0500 amos brooks <atbrooks@snet.net>
writes:
>Hi,
>    DAKO makes a Catalyzed Signal Amplification Kit. This amplifies 
>the signal
>enough to cause an antibody that usually doesnt stain on FFPE 
>(formalin fixed
>paraffin embedded) tissues. The procedure is a bit complicated and 
>rather
>expensive but the results can be quite quite good.
>    Ventana also makes a one step amplification reagent that I've 
>heard is good
>although I've not used it much.
>Amos Brooks
>
>Jay Turner wrote:
>
>> I am staining a fractured rat femur with an immunohistochemical 
>stain to
>> IGF-1.  The stain is a standard biotin-avidin peroxidase with DAB.   
>The
>> samples were decalcified in Cal-Ex (Fisher), it was a little harsh.  
>There
>> is stain in the section but it is very faint.  I don't want to re-do 
>these
>> animals and re-decalcify.  I have tried to use a metal enhanced DAB 
>(cobalt)
>>   but the color is too close to the hematoxylin counterstain.  Is 
>there any
>> other way to enhance DAB and the staining?  Any suggestions?  Is
>> fluorescence the way to go?
>>
>> Thanks!
>>
>> ______________________________________________________
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>
>
>
>

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