Kimberly Carter wrote:

> Maureen
>     I have not cut  brain sections quite that thick, but  I have cut them up to
> 15 microns. I have cut other types of tissue up to 50 microns. For thicker
> sections, always start with a room temperature block. If you need to, dip a
> clean gauze into your warm water bath and gently wipe it across the tissue.
> Also, you may need to adjust the temperature of your water bath. I usually lower
> the temperature of the water from where I cut my routine blocks. I think once
> you get the hang of it, you will find it actually very easy.
>
> Kim Carter
> OSU Medical Center
> Columbus, Ohio
> Maureen Decorah wrote:
>
> > Received this question but I don't have any experience cutting this thick.
> > Does anyone have suggestions for him?
> >
> >  I am trying to cut 40 micron sections of brain tissue (mouse)
> > >embedded in paraffin.  The sections don't come off the knife well and if I
> > >do get them they crack later.  I seem to be able to get 5 micron sections
> > >from the same block ok.  Is it possible to get 40 micron sections and if so
> > >are there any "tricks" to doing it?  Thanks again for your help.
> > >
> >
> >         ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> >         Maureen Decorah, Research Animal Resources Center
> >         385A Enzyme Institute, 1710 University Avenue
> >         University of Wisconsin - Madison, Madison, WI 53705-4098
> >         (608) 262-0933  Fax(608) 265-2698  E-mail: decorah@rarc.wisc.edu
> >         ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~