Hi Anita,
nope, we donīt check IHC buffer solutions every day. Usually thereīs only a
few days from preparation to use. After preparing we adjust the pH with a
pH-meter and thatīs it.
For more critical buffers like the one buffering the paraformaldehyde for
fixation I like to use phenol-red and usually you see even small deviations
by color-changes.
Good luck and happy staining.

Jochen Schuck

> Von: anita dudley <azdudley@hotmail.com>
> Antworten an: histonet@pathology.swmed.edu
> Datum: Wed, 29 Mar 2000 19:19:17 -0800 (PST)
> An: histonet@pathology.swmed.edu
> Betreff: PH of IHC buffers
> 
> Would anyone out there let me know what they use to check the ph of their
> IHC buffer.  It is a question on the CAP checklist, we have dip sticks but I
> just wonder if other people are using a ph meter and what kind and is it
> checked every day?  thanks so much for the info
> 
> Anita Dudley
> Providence Hosp
> Mobile Ala
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