Re: PH of IHC buffers

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From:Jochen Schuck <>
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Hi Anita,
nope, we donīt check IHC buffer solutions every day. Usually thereīs only a
few days from preparation to use. After preparing we adjust the pH with a
pH-meter and thatīs it.
For more critical buffers like the one buffering the paraformaldehyde for
fixation I like to use phenol-red and usually you see even small deviations
by color-changes.
Good luck and happy staining.

Jochen Schuck

> Von: anita dudley <>
> Antworten an:
> Datum: Wed, 29 Mar 2000 19:19:17 -0800 (PST)
> An:
> Betreff: PH of IHC buffers
> Would anyone out there let me know what they use to check the ph of their
> IHC buffer.  It is a question on the CAP checklist, we have dip sticks but I
> just wonder if other people are using a ph meter and what kind and is it
> checked every day?  thanks so much for the info
> Anita Dudley
> Providence Hosp
> Mobile Ala
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