Hi CMD1352,

this looks like your slides were not perfectly deparaffinized, or the fresh
tissue
dryed out!

Hope it help's,
Joachim

Joachim Siegmund /BTA
Hamburg-Germany


----- Original Message -----
From: <CMD1352@aol.com>
To: <histonet@pathology.swmed.edu>
Sent: Friday, March 17, 2000 1:14 AM
Subject: H & E Staining problems


> Our laboratory uses an automated Leica stainer for H & E.  The pathologist
> has noticed several slides with weak staining around the periphery of the
> tissue section.  The stain is very weak just along the edge of the tissue.
> The rest of the tissue stains fine.  I checked the temperature of the
slide
> dryer and found no problem.  Any ideas how to solve this problem?
>