Re: 10%NBF
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From: | Tony Henwood <henwood@mail.one.net.au> |
To: | histonet <histonet@pathology.swmed.edu>, Katrina Knott <knottk@amc.org> |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Dear Katrina,
> So I made a mistake in making up our 10% neutral buffered formalin. I did
> everything else right, except that I added Potassium Phosphate, dibasic,
> instead of Sodium phosphate dibasic. I made up a lot of this and dont want
> to waste it. What deleterious effect will this cause. Will the tissue
> still fix properly?
>
Take a measured sample, check the pH. Add appropriate NaOH or HCl to
pH to 7+/- 0.2. From this, extrapolate for the total volume of
fixative. The effect should be the same as properly made buffered
formalin but be careful of how much acid/alcohol you add, you might
inadvertantly dilute the formalin too much.
Regards, Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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