Edna,

I would cut them thin; 3-5 um unless there's a reason to have them thicker.

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI  53792-2472


(608)265-6596
FAX: (608)263-1568

> -----Original Message-----
> From:	Edna_J_Gonzalez/Powderject@powderject.com
> [SMTP:Edna_J_Gonzalez/Powderject@powderject.com]
> Sent:	Wednesday, March 22, 2000 10:22 AM
> To:	HistoNet Server
> Subject:	frozen lymph nodes
> 
> 
> 
> I'm planning to cut frozen sections of mouse lymph nodes. What will be the
> best thickness to cut them at? Is 8 or 10 um OK or should I go lower?
> 
> 
>