RE: Richard-Allan Stains

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From:"Weems, Joyce" <JWEEMS@sjha.org>
To:'Linda Jenkins' <jlinda@ces.clemson.edu>, histonet@pathology.swmed.edu
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Dear me, I wouldn't want anyone to think that either, AND I am one of the
sites using them. When Richard Allen talked of removing Hematoxylin 7211
from the market, I was one of the techs kicking and screaming to keep it
alive!! 

I'm desperate to find a solution to the problem tho and will review
anything!!
 
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	Linda Jenkins [SMTP:jlinda@ces.clemson.edu]
	Sent:	Wednesday, March 29, 2000 9:24 AM
	To:	histonet@pathology.swmed.edu
	Subject:	Richard-Allan Stains

		Laurie Colbert  wondered, "Is Richard Allan a common
denominator
	with everyone who is having problems?????"
		My thoughts on this - I have used Richard-Allan hematoxylin,
eosin,
	clarifier, bluing, and cytology stains practically since their
arrival in
	the marketplace (late '70's - mid-eighties?).  The ONLY consistent,
	reliable procedure I've NEVER had to "tweak" in the last 20 years
has been
	my H &E stain - as it is beautiful!  In fact, in 1990, when I was
first
	introduced to the gentleman (Jerry Fredenburg) who was responsible
for the
	development of these wonderful chemicals I immediately hugged him
and said,
	"Thank You, Thank You, Thank You!"  In fact, all of our area labs
have used
	these stains with great results.
		Now, in all fairness, I must add that I use real Xylenes for
	clearing and graded ethanols for dehydration.  Have not been seen
	consistent results with substitutes.
		And, as a final note, I remember thinking that the original
	question that started this query had a processing procedure that
went
	straight from formalin to 100%alcohol.  Isn't this a little odd?
Doesn't
	everyone use graded alcohols?
		Oh, well...I just didn't want techs, who've never used them,
to get
	the wrong impression about Richard-Allan stains.
		Linda


	*********************************
	Linda Jenkins, HT
	Clemson University
	Department of Bioengineering
	864.656.5553
	**********************************



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