We also use Richard Allen stains and are very happy with them.  We also
using the Bluing Reagent, Clarifier and the Clear-Rite 3.

Jennifer MacDonald

> ----------
> From: 	Linda Jenkins[SMTP:jlinda@ces.clemson.edu]
> Sent: 	Wednesday, March 29, 2000 8:23 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	Richard-Allan Stains
> 
> 	Laurie Colbert  wondered, "Is Richard Allan a common denominator
> with everyone who is having problems?????"
> 	My thoughts on this - I have used Richard-Allan hematoxylin, eosin,
> clarifier, bluing, and cytology stains practically since their arrival in
> the marketplace (late '70's - mid-eighties?).  The ONLY consistent,
> reliable procedure I've NEVER had to "tweak" in the last 20 years has been
> my H &E stain - as it is beautiful!  In fact, in 1990, when I was first
> introduced to the gentleman (Jerry Fredenburg) who was responsible for the
> development of these wonderful chemicals I immediately hugged him and
> said,
> "Thank You, Thank You, Thank You!"  In fact, all of our area labs have
> used
> these stains with great results.
> 	Now, in all fairness, I must add that I use real Xylenes for
> clearing and graded ethanols for dehydration.  Have not been seen
> consistent results with substitutes.
> 	And, as a final note, I remember thinking that the original
> question that started this query had a processing procedure that went
> straight from formalin to 100%alcohol.  Isn't this a little odd?  Doesn't
> everyone use graded alcohols?
> 	Oh, well...I just didn't want techs, who've never used them, to get
> the wrong impression about Richard-Allan stains.
> 	Linda
> 
> 
> *********************************
> Linda Jenkins, HT
> Clemson University
> Department of Bioengineering
> 864.656.5553
> **********************************
>