Cynthia,

When we do in-situ, we always get bubbles during the heating step as well.
I was told at one time that this was ok - just try not to get any bubbles
under the coverslip before the heating step.

Laurie Colbert
SJMC
Burbank, CA

-----Original Message-----
From: Cynthia Favara [mailto:cfavara@niaid.nih.gov]
Sent: Monday, March 27, 2000 9:33 PM
To: 'HistoNet Server'
Subject: In-situ hybridization technique


Did in-situ for the first time last week and would like to know how people
keep air bubbles from forming when the sections are heated. Had a terrible
time!
Cynthia Favara 
NIAID/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
PH: 406-363-9317 
FAX: 406-363-9286 

e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov>