IHC control -Reply

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From:Richard Cartun <Rcartun@harthosp.org>
To:CGerorge@optima.org, histonet@pathology.swmed.edu
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We use formalin-fixed liver for a positive control for both of these cytokeratins.  In general, bile ducts and hepatocytes are positive for CAM 5.2, whereas only bile ducts show immunoreactivity with AE1 &AE3.  Please note that hepatocytes can show immunoreactivity for AE1 & AE3, but usually only after harsh pretreatment or when a non-formalin fixative is used.


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