Air dry
Fix in methanol - 20mins!
Stain


Since the stain is in alcohol, some leave out the  fixative stage, on the 'fix as you stain' basis.
To actually advocate (as opposed to having the antipodean, 'she'll be right' attitude) leaving out the fixation, is weird.

Terry L Marshall
Rotherham General Hospital, Yorkshire


-----Original Message-----
From: p=NHS NATIONAL INT;a=NHS;c=GB;dda:RFC-822=DGoodwin(a)CHSNJ.org; 
Sent: 20 March 2000 19:24
To: p=NHS NATIONAL
INT;a=NHS;c=GB;dda:RFC-822=HistoNet(a)Pathology.swmed.edu;
Subject: Fixation of BM smears for Wright/ Giemsa


Good Morning, Histonet!   Happy Spring 2000!

Our lab has always been accustomed to fixing bone marrow smears in
methanol prior to staining for Fe and with Wright -Giemsa.  

We now have a new director who advocates air-drying only.

I have been unable to find a reference for this subject, so I defer to
the think-tank at Histonet.   

Any takers?


Diana Goodwin,  HT
Trenton,  NJ