RE: FW: H & E Staining problems

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From:"Weems, Joyce" <>
To:"'Colbert, Laurie'" <>, "'Bill Sinai (Anatomical Pathology)'" <>,
Content-Type:text/plain; charset="iso-8859-1"

I'm using Richard Allen Hematoxylin 7211 - Shandon's Instant Eosin - and
Stephen's Alcohols and formalin. (which come from Richard Allen through

This will be interesting! Our problem started in 98 also, but we were using
different hematoxylin and eosin then - Surgipath Gills, and Surgipath eosin.

During trials, the docs liked the regressive stain so we continued with the

Anxious to hear if there's a trend. j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta

	-----Original Message-----
	From:	Colbert, Laurie []
	Sent:	Tuesday, March 21, 2000 3:35 PM
	To:	'Bill Sinai (Anatomical Pathology)'; Colbert, Laurie;
	Subject:	RE: FW: H & E Staining problems

	We purchase all of our processing and staining products from Richard
	However, we have been having this problem since December '99 and
have gone
	through many orders of formalin and other reagents.  

	Is Richard Allan a common denominator with everyone who is having

	Laurie Colbert
	SJMC - Burbank, CA

	-----Original Message-----
	From: Bill Sinai (Anatomical Pathology)
	Sent: Monday, March 20, 2000 9:27 PM
	To: Colbert, Laurie;
	Subject: Re: FW: H & E Staining problems

	Date:          Mon, 20 Mar 2000 12:08:00 -0800
	From:          "Colbert, Laurie" <>
	Subject:       FW: H & E Staining problems
	To:            "HistoNet (E-mail)" <>

	Dear Laurie,

	This problem rears its ugly head occasionaly in Australia as well.  
	Nearly every time it can be followed back to a " BAD 
	BATCH of FORMALDEHYDE".  Looking at Histonet lately I 
	feel this is happening to people in the USA.  Are the people having 
	this problem all obtaining supplies from the one 
	supplier or initial source?

	We fixed the problem by discarding our formalin 
	supplies, supplying a new batch and presto most of the 
	problems went away.  We now only have the odd case which 
	causes problems (probably still some of the old batch).  We supply 
	all our customers with formalin pots and concentrated solution from 
	our central site.

	Message-----From: Colbert, Laurie Sent: Friday, March 17, 2000 2:14 
	PM To: '' Subject: RE: H & E Staining problems

	We have had staining and/or processing problems off and on for
years, and we
	are presently going through a very bad period where the problem is
	correcting itself (which it always has in the past).  Our sections
have very
	faded nuclei mainly around the edges and an overall hazy look.
Several times
	the pathologists have commented that the slides were too pink. 

	For every theory we come up with, we can always disprove that
theory.  We do
	histology for five different hospitals, and the blocks are processed
	different processors.  Our processing schedules are pretty much the
same on
	each processor.  But when we have problems, we have problems with
all of the
	hospitals' blocks.

	The main problem is with the small biopsies (skin, GI's, prostate
	biopsies, cervical biopsies).  We think it may be an over-processing
	problem, but when we got a demo processor so that we could set up a
	run for the biopsies, we still had problems.  The tissue looked
better, but
	not great.  One day the slides look as bad as they ever had.  Our
	processing schedule is nine hours long.  We have one formalin, two
	three 100% alcohols, three xylenes, and three paraffins.  We use no
	except on the paraffins.

	We have approached the problem as a staining problem, but have
pretty much
	ruled this out.  When we sent our slides to other facilities for
	they still looked bad.

	We are at a total loss.  Has anyone ever had water problems that
caused this
	problem?  We use tap water.

	Laurie Colbert
	Saint Joseph Medical Center
	Burbank, Ca
	(818) 557-5495 


	-----Original Message-----
	From: []
	Sent: Thursday, March 16, 2000 7:27 PM
	Subject: H & E Staining problems

	Our laboratory uses an automated Leica stainer for H & E.  The
	has noticed several slides with weak staining around the periphery
of the 
	tissue section.  The stain is very weak just along the edge of the
	The rest of the tissue stains fine.  I checked the temperature of
the slide 
	dryer and found no problem.  Any ideas how to solve this problem?

	Bill Sinai
	Department Manager
	Tissue Pathology
	ICPMR Westmead Hospital 
	Phone 61+2+9845 7774  Fax 61+2+9687 2330

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