RE: FW: H & E Staining problems
<< Previous Message | Next Message >>
|From:||"Weems, Joyce" <JWEEMS@sjha.org>|
|To:||"'Colbert, Laurie'" <LColbert@phsca.org>, "'Bill Sinai (Anatomical Pathology)'" <Bills@icpmr.wsahs.nsw.gov.au>, email@example.com|
I'm using Richard Allen Hematoxylin 7211 - Shandon's Instant Eosin - and
Stephen's Alcohols and formalin. (which come from Richard Allen through
This will be interesting! Our problem started in 98 also, but we were using
different hematoxylin and eosin then - Surgipath Gills, and Surgipath eosin.
During trials, the docs liked the regressive stain so we continued with the
Anxious to hear if there's a trend. j
Saint Joseph's Hospital of Atlanta
From: Colbert, Laurie [SMTP:LColbert@phsca.org]
Sent: Tuesday, March 21, 2000 3:35 PM
To: 'Bill Sinai (Anatomical Pathology)'; Colbert, Laurie;
Subject: RE: FW: H & E Staining problems
We purchase all of our processing and staining products from Richard
However, we have been having this problem since December '99 and
through many orders of formalin and other reagents.
Is Richard Allan a common denominator with everyone who is having
SJMC - Burbank, CA
From: Bill Sinai (Anatomical Pathology)
Sent: Monday, March 20, 2000 9:27 PM
To: Colbert, Laurie; firstname.lastname@example.org
Subject: Re: FW: H & E Staining problems
Date: Mon, 20 Mar 2000 12:08:00 -0800
From: "Colbert, Laurie" <LColbert@phsca.org>
Subject: FW: H & E Staining problems
To: "HistoNet (E-mail)" <email@example.com>
This problem rears its ugly head occasionaly in Australia as well.
Nearly every time it can be followed back to a " BAD
BATCH of FORMALDEHYDE". Looking at Histonet lately I
feel this is happening to people in the USA. Are the people having
this problem all obtaining supplies from the one
supplier or initial source?
We fixed the problem by discarding our formalin
supplies, supplying a new batch and presto most of the
problems went away. We now only have the odd case which
causes problems (probably still some of the old batch). We supply
all our customers with formalin pots and concentrated solution from
our central site.
Message-----From: Colbert, Laurie Sent: Friday, March 17, 2000 2:14
PM To: 'CMD1352@aol.com' Subject: RE: H & E Staining problems
We have had staining and/or processing problems off and on for
years, and we
are presently going through a very bad period where the problem is
correcting itself (which it always has in the past). Our sections
faded nuclei mainly around the edges and an overall hazy look.
the pathologists have commented that the slides were too pink.
For every theory we come up with, we can always disprove that
theory. We do
histology for five different hospitals, and the blocks are processed
different processors. Our processing schedules are pretty much the
each processor. But when we have problems, we have problems with
all of the
The main problem is with the small biopsies (skin, GI's, prostate
biopsies, cervical biopsies). We think it may be an over-processing
problem, but when we got a demo processor so that we could set up a
run for the biopsies, we still had problems. The tissue looked
not great. One day the slides look as bad as they ever had. Our
processing schedule is nine hours long. We have one formalin, two
three 100% alcohols, three xylenes, and three paraffins. We use no
except on the paraffins.
We have approached the problem as a staining problem, but have
ruled this out. When we sent our slides to other facilities for
they still looked bad.
We are at a total loss. Has anyone ever had water problems that
problem? We use tap water.
Saint Joseph Medical Center
From: CMD1352@aol.com [mailto:CMD1352@aol.com]
Sent: Thursday, March 16, 2000 7:27 PM
Subject: H & E Staining problems
Our laboratory uses an automated Leica stainer for H & E. The
has noticed several slides with weak staining around the periphery
tissue section. The stain is very weak just along the edge of the
The rest of the tissue stains fine. I checked the temperature of
dryer and found no problem. Any ideas how to solve this problem?
ICPMR Westmead Hospital
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774 Fax 61+2+9687 2330
<< Previous Message | Next Message >>