I am looking for any recommendations with regards to
processing mouse brain tissue.  Our lab currently uses
the same program for processing mouse tissue that is
used for processing tissue from human and other
mammals.  The problem that I have is that when cutting
the tissue it is often to dry to get good sections
without resorting using ammonia water to rehydrate the
tissue every couple of sections.  This can be time
consuming and I would like to add a second program to
our tissue processor that would not dry the tissue
out.  If anyone has done this in their lab and can
recommend a protocol it would greatly be appreciated. 
Thanks for your help.

Daniel Martinez
Center for Neurodegenerative Disease Research
University of Pennsylvania
nativenewjersian@yahoo.com


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