A doctor has asked our lab to do a double immuno stain using both p21 and ki 67.
My question is---where can I look for a relatively easy to understand method to
accomplish this?  I suspect that I will need to use 2 different chromagens(dab
and aec) but do I have to retreive the antigens twice, block peroxidase twice,
etc?
My assumption is that I should proceed with one antibody stain form start to
finish and then for the second antibody I should start the procedure from the
point of primary antibody application to the second chromagen.
Any help anybody can give regarding either the method or where to look up the
method would be greatly appreciated.
Sincerely,
Christine Klein
MD Anderson Cancer Center
Houston, Tx.