40 micron sections

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From:Maureen Decorah <decorah@rarc.wisc.edu>
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Received this question but I don't have any experience cutting this thick.
Does anyone have suggestions for him?

 I am trying to cut 40 micron sections of brain tissue (mouse)
>embedded in paraffin.  The sections don't come off the knife well and if I
>do get them they crack later.  I seem to be able to get 5 micron sections
>from the same block ok.  Is it possible to get 40 micron sections and if so
>are there any "tricks" to doing it?  Thanks again for your help.

	Maureen Decorah, Research Animal Resources Center
	385A Enzyme Institute, 1710 University Avenue
	University of Wisconsin - Madison, Madison, WI 53705-4098
	(608) 262-0933  Fax(608) 265-2698  E-mail: decorah@rarc.wisc.edu

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