Dear Histonetters:
		Can someone provide information as to why most IHC protocols have you
drain off the blocking solution before adding the primary and not rinsing
with buffer in between?  In all the procedures I have looked at they
explain the theory behind the other steps but not this one.  Thanks in
advance.

						Tina


Tina Van Meter
Dept. of Neuroscience
The Ohio State University
Columbus, Ohio