Fixatives for crow and Avian Tissue
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From: | Gwin Kerce <gkerce@arches.uga.edu> |
To: | histonet <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
We are just doing light microscopy. It is not the ultrastructure that is the
trouble ( my mistake). The formalin fixation
does not have the crips detail that is desired. We are working on a study of
35 week old leghorns ( male obviously).
The calcium deposit very in size and the tissue is sectioned undecalcified.
> Our lab is working on a project that involves locating calcium deposits
> in avian testis and epididymis. We have tried to use Bouin's Solution,
> unfortunately,
> it dissolves the deposits of interest. We also tried 10% Buffered
> Foramlin, however it lacks the desired crispness. Does anyone
> have any suggestions for a fixative? We need to fix both the testicular
> and epididmyal tissue.
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