Re: Fixation time for mouse skin

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From:Robert Schoonhoven <>
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What, precisely, did you do when you "dipped in paraffin"?  Did you
immerse them in several changes of paraffin wax and allow the wax to
infiltrate the tissues? wrote:
> I am asking this question for a graduate student here in our lab...Some mouse
> skin was recently processed and when trying to cut the sections it was found
> that the tissue was too brittle.  The skins were fixed in 10% nbf for 16 hours
> and then dehydrated in a series of ethanol dilutions(70%, 85%, 90% and 100%) for
> 20 minutes each.  Skin was then cleared in xylene for 20minutes, dipped in
> parrafin and then embedded.  We think that perhaps the skin was fixed for too
> long a period.  Does anyone have any suggestions regading times for the
> fixation, clearing or parrafin infiltraion?
> I realize that this question has probably been asked many times before, but I am
> relatively new to the field of histology and am still learning where to find
> good accurate information.
> Thanking you in advance,
> I remain sincerely yours,
> Christine Klein
> MD Anderson Cancer Center Houston Texas

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first. 
Mark Twain [Samuel Langhornne Clemens] (1835-1910)

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