cell pellets for IHC/ISH

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From:"Connolly, Brett" <brett_connolly@merck.com>
To:"'histonet'" <histonet@Pathology.swmed.edu>
Date:Thu, 24 Jun 1999 11:17:28 -0400

I looking for advice on proper fixing/freezing of cell pellets for cryostat
sectioning and subsequent fluorescent IHC and ISH. These will be separate
populations of monocytes, PMNs, eosinophils, and lymphocytes from peripheral
blood and we'll be staining for monos (CD68), PMNs (CD18,) T & B cell
markers, other various cytokines and chemokine receptors. Your
suggestions/secrets and protocols will be greatly appreciated. Should 4%
paraformaldehyde be avoided?


Brett M. Connolly, Ph.D.
Senior Research Biologist
Dept. of Human Genetics
Merck Research Laboratories
PO Box 4
West Point, PA 19486
tel. 215-652-2501
FAX 215-652-2075

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