Forwarded message:
>From HistoNet@Pathology.swmed.edu Thu Jun 24 10:55:18 1999
Date: Thu, 24 Jun 1999 10:04:48 -0400 (EDT)
Message-Id: <199906241404.KAA19588@pilot008.cl.msu.edu>
From: "David John Schmitz" <schmitzd@pilot.msu.edu>
Subject: axolotl 
To: histonet@Pathology.swmed.edu
Content-Type: text/plain

Hi  everyone,

I am having a few problems with my histology.  I am trying to obtain a stage
series of axolotl embryos and I am having trouble because the have a tremendous
amount of fatty Yolk filled cells.  I have tried using bouinUs fix and smithUs
fix, and smithUs fix seems to work the best on the yolk. But, now I have a
number of embryos that have been fixed in buffered formalin, Can I postfix them
with SmithUs and have I work out OK?  If you have any other ideas of dealing
with the yolk problem, let me know .
I am also having a problem orientating the very early stage embryos and I heard
that agarose can be use for this.  Dose anyone know how to use agarose?
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