Re: Immunoperoxidase Slide Drying

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From:Tim Morken <timcdc@hotmail.com>
To:histonet@Pathology.swmed.edu
Reply-To:
Date:Thu, 17 Jun 1999 20:45:17 -0400 (EDT)
Content-Type:text/plain; format=flowed


If you want to use the slides as soon as possible then be sure that there is 
little or no water under the section. I always give the slide a good shake 
right after draining off the water. this gets rid of any excess water under 
the section. Then put the slides in a 37 deg oven or on a 37 deg hot plate 
for 30 to 60 minutes. With Plus slides this is all you will usually need to 
dry the water and adhere the tissue to the slide.


Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043



----Original Message Follows----
From: Richard Maliniak <maliniakrm@worldnet.att.net>
To: HistoNet <histonet@pathology.swmed.edu>
Subject: Immunoperoxidase Slide Drying
Date: Thu, 17 Jun 1999 13:42:01 -0700

We are trying to reduce the turn around time on our immunostains.  I'd
like to get an idea of how most people dry their sections on positively
charged slides in diagnostic laboratories.  Time?  Temperature?
Convection Oven?  Microwave Oven?






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