Dear Histonet Folks,

Just a note pertaining to the recent discussions about using a waterbath at
80C for antigen retrieval. Several years ago I ran across an article in
Applied Immunochemistry which uses an overnight incubation in a regular lab
oven set at 75C. We use this to great advantage in our lab.We simply
deparaffinize our slides late in the day, make up the citrate buffer and
throw them in the oven overnight. We use the old Ventana ES and can easily
set up several runs which are then ready to pop on the stainer the next
morning. This method is very gentle on all of our tissue but is especially
nice for mammary tissue. We prefer this over any other method. One note
though, it cannot be used with EDTA which is much too harsh.

The reference is Yan-gao Man, M.D., and Fattaneh A. Tavassoli, M.D.   4(2):
139-141  1996

Sincerely 

Mary Ann
Mary Ann Miller
University of Cincinnati
College of Medicine  ML 529
231 Bethesda Avenue
Cincinnati, Ohio  45267
FAX: (513) 558-2289