Dear Michelle,

We are responding to your posted Histonet inquiry about the Vector 
NovaRED peroxidase substrate. The general dehydration and clearing 
procedure employed here at Vector Labs is as follows:

After substrate development and rinsing, the slides are placed into 2 
changes of 95% alcohol for 2 min each, then directly into 2 changes of 
100% alcohol for 2 min each, then into 2 or 3 changes of xylene (or 
suitable substitute) for about 2 mins each. The preparations are then 
mounted with a compatible mounting media.

Using this above mentioned protocol, we have not experienced any problems 
with the Vector NovaRED substrate dissolving. We would not recommend 
using recycled alcohol in the 95% dehydration steps, however, denatured 
alcohol will be fine. Extended incubations in the alcohols (greater than 
30 min) or lower alcohols (50% and 70%) may adversely effect the color 
intensity. 

The photographic example of Vector NovaRED staining we have provided in 
our latest catalog and newsletter (Vectagram), was taken from a section 
which had been processed through this described method.

We hope this information is of help to you, and please contact the Vector 
Laboratories technical service department should you require further 
assistance.

Sincerely,


Technical Services

Vector Laboratories
30 Ingold Road
Burlingame, CA 94010

Tel:  (650) 697-3600
Fax:  (650) 697-0339
E-mail:  vector@vectorlabs.com
Website:  http://www.vectorlabs.com