decrease in iron in BM bx?

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From:Francie Gallery <>
Date:Tue, 15 Jun 1999 11:13:00 -0400
Content-Type:text/plain; charset=us-ascii

A question was posed to me this morning for the HistoNet folks.

     bone marrow biopsies fixed in 10% NBF, decalcified in 5% formic acid
for 4-8hrs then (routine) processed in paraffin
     there is a marked decrease in iron in these BM bx specimens compared
to that of the associated clots and aspirates (prepared as above except for
the decal)
     1)  has anyone else experienced this (or similar) observation?
     2)  ideas for remedying this issue?

Thanks in advance!

Department of Neurobiology & Behavior, SUNY at Stony Brook, Stony Brook, NY
Histology Department, Suffolk County Medical Examiner's Office, Hauppauge,

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