A question was posed to me this morning for the HistoNet folks.

History:
     bone marrow biopsies fixed in 10% NBF, decalcified in 5% formic acid
for 4-8hrs then (routine) processed in paraffin
Observation:
     there is a marked decrease in iron in these BM bx specimens compared
to that of the associated clots and aspirates (prepared as above except for
the decal)
Questions:
     1)  has anyone else experienced this (or similar) observation?
     2)  ideas for remedying this issue?


Thanks in advance!
~Francie~

FGALLERY@neurobio.sunysb.edu

Department of Neurobiology & Behavior, SUNY at Stony Brook, Stony Brook, NY
Histology Department, Suffolk County Medical Examiner's Office, Hauppauge,
NY