Storage of Frozen Sections!

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From:jennifer.hoover@pharma.Novartis.com
To:Histonet@Pathology.swmed.edu
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Date:Wed, 09 Jun 1999 11:12:23 -0400
Content-Type:text/plain; charset=us-ascii

     Good Morning Histonetters!  Does anyone have experience with storage of
sectioned frozen tissue???  In the past I have only sectioned tissue that I
would immunostain immediately.  Currently my boss is concerned with losing
tissue each time that I would re-trim a block; thus he prefers that I section
approximately 20 slides at a time per block and store the slides for subsequent
immunohistochemical staining (to be performed ASAP).  I consulted one book which
suggested that fixed slides could be stored at -70 C if dessicated!  Any
opinions or experience in this matter???  Thank you very much in advance!


Jennifer Hoover
Histologist
Transplantation Biology
Novartis Pharmaceuticals
Summit, NJ





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