Storage of Frozen Sections!
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From: | jennifer.hoover@pharma.Novartis.com |
To: | Histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 09 Jun 1999 11:12:23 -0400 |
Content-Type: | text/plain; charset=us-ascii |
Good Morning Histonetters! Does anyone have experience with storage of
sectioned frozen tissue??? In the past I have only sectioned tissue that I
would immunostain immediately. Currently my boss is concerned with losing
tissue each time that I would re-trim a block; thus he prefers that I section
approximately 20 slides at a time per block and store the slides for subsequent
immunohistochemical staining (to be performed ASAP). I consulted one book which
suggested that fixed slides could be stored at -70 C if dessicated! Any
opinions or experience in this matter??? Thank you very much in advance!
Jennifer Hoover
Histologist
Transplantation Biology
Novartis Pharmaceuticals
Summit, NJ
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