Re: cryosectioning questions

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From:Alan Bright <>, "Karen D. Larison" <>
Date:Thu, 03 Jun 1999 11:49:58 +0100
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Dear Karen,

Please would you answer the following questions to assist me to determine
the most suitable way to suit your needs in cryosectioning:-

1) Types of tissues you need to section?

2) Thickness ranges required?

3) Largest specimen size in what tissue?

4) Temperature range required?

The reason for asking these questions will outline if it is possible to
achieve all your sectioning on disposable blades or if it would be better to
stick with conventional knives.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margarets Way
PE18 6EB

Tel No; 01480 454528
Fax No;01480 456031
Email ;

-----Original Message-----
From: Karen D. Larison <>
To: <>
Date: Tuesday, June 01, 1999 07:29
Subject: cryosectioning questions

>I have some cryosectioning questions.  I have a couple of ancient
cryostats, and
>am in the market for a new one, ie one with a disposable blade holder, etc.
>I've had no expereince with disposables, I'm wondering if there are any
>reasons to use your C profile blades for cryosectioning if you have
>capabilities?  Are there some applications where C profiles are still
>for cryosectioning, and how do the two blade types compare for
>Also, if money weren't an issue (if we could all be so lucky!), and you
>afford to buy a cryostat with as many compressors required to cut large
>are there any compelling reasons to continue to use a sliding microtome?
Does it
>have any advantages over cryosectioning in a well equipped cryostat?
>You guys are so wonderful!  I've learned so much by tuning in to this list
>Ever greatful in Oregon,
>Karen Larison

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