Re: Preparation of Zebra Fish

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From:"Karen D. Larison" <LARISONK@UONEURO.uoregon.edu>
To:histoNet@Pathology.swmed.edu
Reply-To:
Date:Fri, 04 Jun 1999 08:59:11 -0800
Content-Type:

There's a nice little BioTechniques article that Gayle turned me onto on 
processing 7-d zebrafish in agarose-embedded arrays.  I have yet to try it, but 
you might want to check it out:  BioTechniques 25:614-618 (1998).  -Karen at 
University of Oregon, birthplace of the zfish as a genetic model for vertebrate 
development


Date:          Thu, 03 Jun 1999 21:33:16 -0400
From:          Histo-Scientific Research Laboratory <histosci@shentel.net>
Subject:       Preparation of Zebra Fish
To:            histoNet@pathology.swmed.edu

Anita,

We recently prepared slides of two week old zebras.  They were about as
small as a fish can be!  We fixed them in 4% paraform. for 24 hours.  Then
we processed them on our regular processing schedule.  Paraffin embedded
them (saggitally as per request from client).  Microtomed them at 4
microns -12 step sections throughout to achieve most vital organs.  H+E
stained on regular schedule and PRESTO- they were beautiful.  Hope this
helps.

Sincerely,

Tom Galati
Histo-Scientific Research Labs.
P.O. Box 30
107 Killmon Road
Basye, VA  22810
(540)856-2222
histosci@shentel.net






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