Re: Preparation of Zebra Fish

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From:"Karen D. Larison" <>
Date:Fri, 04 Jun 1999 08:59:11 -0800

There's a nice little BioTechniques article that Gayle turned me onto on 
processing 7-d zebrafish in agarose-embedded arrays.  I have yet to try it, but 
you might want to check it out:  BioTechniques 25:614-618 (1998).  -Karen at 
University of Oregon, birthplace of the zfish as a genetic model for vertebrate 

Date:          Thu, 03 Jun 1999 21:33:16 -0400
From:          Histo-Scientific Research Laboratory <>
Subject:       Preparation of Zebra Fish


We recently prepared slides of two week old zebras.  They were about as
small as a fish can be!  We fixed them in 4% paraform. for 24 hours.  Then
we processed them on our regular processing schedule.  Paraffin embedded
them (saggitally as per request from client).  Microtomed them at 4
microns -12 step sections throughout to achieve most vital organs.  H+E
stained on regular schedule and PRESTO- they were beautiful.  Hope this


Tom Galati
Histo-Scientific Research Labs.
P.O. Box 30
107 Killmon Road
Basye, VA  22810

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