On Fri, 4 Jun 1999, Willis, Donna wrote:

> Those that have used the DAKO Anti-Human ALK Protein would you please answer
> a couple of questions.
> 
> 1.  What control tissue are you using?
> 
> 2.  What type of detection are you using?
> 
> We are using Ventana Nexes instruments.  After talking to DAKO they
> suggested using the High Intensity Detection.  Has anyone tried this?
> 
> Thanks,
> Donna Willis
> Histology Lab Manager
> Harris Methodist Fort Worth, Texas
> 817-878-5644 office
> 817-878-5698 fax
> email: donnawillis@hmhs.com 
> 

Donna,
I too am having trouble with this antibody and I am using the Dako
Autostainer and all of Dako's reagents.  We're waiting to hear from the
lab at Sloan-Kettering Memorial Hospital in New York City who supposedly
have it going.  I'll let you know what happens.

Dana Settembre
Immunohistochemistry Lab
University Hospital
Newark, NJ