Re: Re: [Histonet] Saturated Fat Staining


Any precise protocol for Fat tissue staining?

The processing procedure?
to remove fat, before immunostaining?



发件人: Judith L. Williams 
发送时间: 2008-07-18  00:31:50 
收件人: Griffin-Reyes, Michelle A 
主题: Re: [Histonet] Saturated Fat Staining 
Hi- Check in to Nile Red or Nile blue if you do not mind doing fluorescent microscopy. It works well on saturated fats and is quite specific. good luck.
Judy Williams
Comparative Medicine
U of Washington
On Fri, 11 Jul 2008, Griffin-Reyes, Michelle A wrote:
> I am having some troubles finding a way to be able to stain saturated
> lipids in FFPE mouse tissues. Osmium tetroxide and oil red O stain well
> but they are only showing unsaturated lipids. My lab is looking to see
> if there is a difference in lipid content of treated mice vs. control
> mice. So far, we have seen very little variability in the amount of
> unsaturated lipids during quantification which leads us to believe there
> may be a difference in saturated fats (based off of human results that
> we have our mouse model based off of). Does anyone have any suggestions?
> Thanks so much for the help!
> Michelle A. Griffin-Reyes
> Comparative Pathology Laboratory
> University of Iowa: College of Medicine
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Judith Williams, PhD, HT(ASCP)
Research Scientist
Department of Comparative Medicine
University of Washington
Seattle, WA 98195
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