Any precise protocol for Fat tissue staining?
The processing procedure?
to remove fat, before immunostaining?
发件人： Judith L. Williams
发送时间： 2008-07-18 00:31:50
收件人： Griffin-Reyes, Michelle A
主题： Re: [Histonet] Saturated Fat Staining
Hi- Check in to Nile Red or Nile blue if you do not mind doing fluorescent microscopy. It works well on saturated fats and is quite specific. good luck.
U of Washington
On Fri, 11 Jul 2008, Griffin-Reyes, Michelle A wrote:
> I am having some troubles finding a way to be able to stain saturated
> lipids in FFPE mouse tissues. Osmium tetroxide and oil red O stain well
> but they are only showing unsaturated lipids. My lab is looking to see
> if there is a difference in lipid content of treated mice vs. control
> mice. So far, we have seen very little variability in the amount of
> unsaturated lipids during quantification which leads us to believe there
> may be a difference in saturated fats (based off of human results that
> we have our mouse model based off of). Does anyone have any suggestions?
> Thanks so much for the help!
> Michelle A. Griffin-Reyes
> Comparative Pathology Laboratory
> University of Iowa: College of Medicine
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Judith Williams, PhD, HT(ASCP)
Department of Comparative Medicine
University of Washington
Seattle, WA 98195
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