Re: [Histonet] Filter paper

I sometimes use coffee filters.
------Original Message------
From: Gayle Callis
To: Mylène de Champlain
Subject: Re: [Histonet] Filter paper
Sent: Jul 24, 2008 4:22 PM

What is important is not the number so much as if the paper is high
retention and what is filtering speed.  Whatman #4, if I remember  
is a medium fast paper, and should be  ideal for filtering staining
solution.  Somewhere in the passage of time, I was told not to use paper
that was really slow filtering speed and high retention for filtering  
solutions as the paper may retain too much of the dye needed. Now  
that could
be an old wife's tale, but to this day I always use a medium fast paper.

   I also purchase something other than Whatman, as companies sell
equivalents for a much cheaper price.  Whatman 54 should also work.    
companies like ThermoScientific give the equivalents for their brand as
compared to Whatman papers and are certainly more economical.

Gayle M. Callis

----- Original Message -----
From: "Mylène de Champlain" 
Sent: Wednesday, July 23, 2008 4:31 PM
Subject: [Histonet] Filter paper


   To filter blue celestin, is what I have to use a filter paper  
number 1 or
4? Thank!

   Mylène de Champlain

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