What is important is not the number so much as if the paper is high  
retention and what is filtering speed.  Whatman #4, if I remember  
correctly, is a medium fast paper, and should be  ideal for filtering  
staining solution.  Somewhere in the passage of time, I was told not  
to use paper that was really slow filtering speed and high retention  
for filtering dye solutions as the paper may retain too much of the  
dye needed. Now that could be an old wife's tale, but to this day I  
always use a medium fast paper.

  I also purchase something other than Whatman, as companies sell  
equivalents for a much cheaper price.  Whatman 54 should also work.    
Big companies like ThermoScientific give the equivalents for their  
brand as compared to Whatman papers and are certainly more economical.

Gayle M. Callis
HTL/HT/MT(ASCP)


----- Original Message ----- From: "Mylène de Champlain"  

To: 
Sent: Wednesday, July 23, 2008 4:31 PM
Subject: [Histonet] Filter paper



Hi,

  To filter blue celestin, is what I have to use a filter paper  
number 1 or 4? Thank!

  Mylène de Champlain
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