What type of tissue are you trying to trace?  If fixed, immature rodent tissue, have you considered one of the fluorescent tracers such as DiI, DiO, DiD, etc. available through Molecular Probes (Invitrogen)?  DiI (red fluorescence) is the brightest.  These dyes don't work well in adult tissue, however.

Rivka

Rivka A. Rachel, MD, PhD
Staff Scientist, National Eye Institute
Neurobiology-Neurodegeneration and Repair Laboratory
Tel: 301 443-4906



-----Original Message-----
From: tf [mailto:tifei@foxmail.com]
Sent: Fri 7/11/2008 11:41 AM
To: histonet
Subject: [Histonet] Anterograde tracing
 
I just want to discuss the best anterograde tracer.

BDA, PHA-L, CTB-FITC, CTB-Rhodamine, WGA-HRP, HRP, and viral vectors all could be anterograde tracer.
However, BDA is diffusible like all fluroscent dyes, does not have the necessary direction.
CTB-conjugated sereis have limited diffusion ability when injected with pressure, just like PHA-L, thus are not good in labeling a larger group of neurons when single rather multiple injections is required.
WGA-HRP & HRP both antero & retrogradely transported, HRP & virus even transynaptically.

So, any one has other ideas? Such as shorten the period after injection to reduce the diffusion, or using immunodetection to visualize the CTB-FITC, for example?

2008-07-11 



tf 
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