VWR has the metal slide trays  #48469-004----- Original Message ----From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.eduSent: Tuesday, July 8, 2008 8:06:49 AMSubject: Histonet Digest, Vol 56, Issue 9Send Histonet mailing list submissions to    histonet@lists.utsouthwestern.eduTo subscribe or unsubscribe via the World Wide Web, visit    http://lists.utsouthwestern.edu/mailman/listinfo/histonetor, via email, send a message with subject or body 'help' to    histonet-request@lists.utsouthwestern.eduYou can reach the person managing the list at    histonet-owner@lists.utsouthwestern.eduWhen replying, please edit your Subject line so it is more specificthan "Re: Contents of Histonet digest..."Today's Topics:  1. Metal Slide Trays (Metzger, Kenneth)  2. RE: H&E quality check (Christine Bark)  3. RE: Metal Slide Trays (Bartlett, Jeanine (CDC/CCID/NCZVED))  4. Vector Labs Nova HRP--Red Substrate: Not Really EtOH      compatible? (JR R)  5. Help needed for brain processing (Shirley Powell)=0A  6. Antibody for the detection of human cells needed (Alexandra Meinl)  7. tissue processor (Atoska Gentry)  8. Re: Gomeri Iron Stain (Maxim_71@mail.ru)  9. Leitz 1512 rotary microtome (sharon.osborn@comcast.net)  10. RE: Antibody for the detection of human cells needed      (Liz Chlipala)  11. Re: H&E quality check (Robert Richmond)  12. Re: H&E quality check (Angela Bitting)  13. RE: Re: H&E quality check (Michael Mihalik)  14. HT position available (LINDA MARGRAF)  15. RE: H&E quality check (Weems, Joyce)  16. RE: Re: H&E quality check.    . (Henry, Charlene)  17. RE: Re: H&E quality check.    . (Michael Mihalik)  18. RE: Gomeri Iron Stain (Tony Henwood)  19. Re: H&E quality check. (Dave & Lee Mayhew)  20. RE: Re: H&E quality check. (Michael Mihalik)  21. Re: Re: H&E quality check. (Dave & Lee Mayhew)  22. Re: Re: H&E quality check. . (Anne van Binsbergen)=C2  23. Re: H&E quality check (Rene J Buesa)  24. RE: Re: H&E quality check. . (Cheri Miller)----------------------------------------------------------------------Message: 1Date: Mon, 7 Jul 2008 11:33:15 -0600From: "Metzger, Kenneth" Subject: [Histonet] Metal Slide TraysTo: Message-ID:    Content-Type: text/plain;  Â=A0 charset="iso-8859-1"Does anyone know where I can order the 20 slot metal slide trays? Thanks,kenKen Metzger HTL(ASCP)Histology SupervisorARUP Laboratories500 Chipeta waySalt Lake City, UT 84108801.583.2787 ext 3101- ------------------------------------------------------------------The information transmitted by this e-mail and any includedattachments are from ARUP Laboratories and are intended only for therecipient. The information contained in this message is confidentialand may constitute inside or non-public information underinternational, federal, or state securities laws, or protected healthinformation and is intended only for the use of the recipient.Unauthorized forwarding, printing, copying, distributing, or use ofsuch information is strictly prohibited and may be unlawful. If youare not the intended recipient, please promptly delete this e-mailand notify the sender of the delivery error or you may call ARUPLaboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1(800) 522-2787 ext. 2100------------------------------Message: 2Date: Mon, 7 Jul 2008 10:37:27 -0700From: "Christine Bark" Subject: [Histonet] RE: H&E quality checkTo: histonet@lists.utsouthwestern.eduMessage-ID:=0A    Content-Type: text/plain; charset=us-asciiWe check several different slides from our first rack of the day for H&Eand cutting quality.Christine Bark HT(ASCP)Senior Histotech, PathologySaddleback Memorial Medical Center949-452-3548cbark@memorialcare.org=0A-----Original Message-----From: "Angela Bitting" Subject: [Histonet] H&E quality checkJust curious as to how other hospital labs quality check their H&Eslides? Do most review a percentage microscopically? ______________________________________________________________________________Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.------------------------------Message: 3Date: Mon, 7 Jul 2008 13:40:13 -0400From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Metal Slide TraysTo: "Metzger, Kenneth" ,    histonet@lists.utsouthwestern.edu=0AMessage-ID:    <1CE1847DFEA0A647B1CCDE4108EA60A7F23C7E@LTA3VS011.ees.hhs.gov>Content-Type: text/plain; charset=us-ascii=0AI think this is what you are looking for.  http://www.daigger.com/catalog/product?deptId=&prodId=16050Jeanine BartlettInfectious Diseases Pathology Branch(404) 639-3590 jeanine.bartlett@cdc.hhs.gov-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger,KennethSent: Monday, July 07, 2008 1:33 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Metal Slide Trays=0ADoes anyone know where I can order the 20 slot metal slide trays?Thanks,kenKen Metzger HTL(ASCP)Histology SupervisorARUP Laboratories500 Chipeta waySalt Lake City, UT 84108801.583.2787 ext 3101- ------------------------------------------------------------------The information transmitted by this e-mail and any included attachmentsare from ARUP Laboratories and are intended only for the recipient. Theinformation contained in this message is confidential and may constituteinside or non-public information under international, federal, or state=0Asecurities laws, or protected health information and is intended onlyfor the use of the recipient.Unauthorized forwarding, printing, copying, distributing, or use of suchinformation is strictly prohibited and may be unlawful. If you are notthe intended recipient, please promptly delete this e-mail and notifythe sender of the delivery error or you may call ARUP LaboratoriesCompliance Hot Line in Salt Lake City, Utah USA at (+1=0A(800) 522-2787 ext. 2100_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 4Date: Mon, 7 Jul 2008 10:54:49 -0700From: JR R Subject: [Histonet] Vector Labs Nova HRP--Red Substrate: Not Really    EtOH Â=A0  compatible?To: Message-ID: Content-Type: text/plain; charset="Windows-1252"The Product information says: "NovaREDâ„¢ substrate may bedehydrated in ethanol and cleared in organic solvents such as xyleneand permanently mounted in a non-aqueous mounting medium such asVectaMount"But I have done two stains now where I initially had intense, specific staining at first--But after my slides come out of the EtOH/Xylene for coverslipping, the staining has vanished.=0AThis is getting to be expensive!Has anyone else ever had this problem with Nova Red?  Any ideas?Jerry RicksResearch ScientistUniversity of WashingtonDepartment of Pathology_________________________________________________________________Need to know now? Get instant answers with Windows Live Messenger.http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_messenger_072008------------------------------Message: 5Date: Mon, 07 Jul 2008 14:07:27 -0400From: Shirley Powell Subject: [Histonet] Help needed for brain processingTo: histonet@lists.utsouthwestern.eduMessage-ID: <01MWVKZ3Y6Y200ANAP@Macon2.Mercer.edu>Content-Type: text/plain; charset=us-asciiA friend of mine, who has been banned from histonet by a micro-managingemployer, needs advice from those who use the microwave to expedite fixationof whole or partial brains for routine processing, or any other rapid meansof getting complete fixation on brain. Please send all responses to mepersonally at this address and I will forward the information to them.  Thanks in advance for your help.Shirley Powell------------------------------=0AMessage: 6Date: Mon, 7 Jul 2008 20:24:22 +0200From: "Alexandra Meinl" Subject: [Histonet] Antibody for the detection of human cells neededTo: histonet@lists.utsouthwestern.eduMessage-ID:    Content-Type: text/plain; charset=UTF-8Hi all,=0AI'm searching for an antibody for the detection of human cells in mousetissue.And: we don't know what type of cells we're trying to find.=0AI thought about an ab like a-hu-nuclear membrane or GM 130.Does anybody this? What antibody would you recommend?Any suggestions greatly appreaciated!Alexandra------------------------------Message: 7Date: Mon, 07 Jul 2008 13:35:13 -0500From: Atoska Gentry Subject: [Histonet] tissue processorTo: Histonet Message-ID: <487261E1.3020101@vetmed.auburn.edu>Content-Type: text/plain; charset=ISO-8859-1; format=flowed=0Ahello, please will someone who has info on a modern tissue processor =0Acomparable to an obsolete  25 yr.Autotechnicon Ultra II Tissue Processor please contact me with specifications, product info & pricing ASAP? Thanks, Atoska------------------------------Message: 8Date: Mon, 7 Jul 2008 22:30:20 +0400From: Maxim_71@mail.ruSubject: Re: [Histonet] Gomeri Iron StainTo: histology@gradymem.orgCc: histonet@lists.utsouthwestern.eduMessage-ID: <228377341.20080707223020@mail.ru>Content-Type: text/plain; charset=us-asciiAngie:We never used MWO modification for Gomori Iron Stain.Iron is beautifully can demonsrtate at RT for 10 mins.Vapours of cyanid is very toxic for personnel.=0AMaxim PeshkovRussia,Taganrog.------------------------------Message: 9Date: Mon, 07 Jul 2008 19:11:41 +0000From: sharon.osborn@comcast.netSubject: [Histonet] Leitz 1512 rotary microtomeTo: histonet@lists.utsouthwestern.eduMessage-ID:  Â=A0 <070720081911.18025.48726A6D0006F323000046692215555884029D010D9C01D202019D0E089C@comcast.net>    Histonetters,  =C2    I have a copy of the instructions manual for above referenced microtome should anyone still have this workhorse and need the instructions.  Please send me your contact information and I am happy to send out.  If there is more than one person requesting it, I can make copies.=0Asharon osbornLab VisionThermo FisherFremont, CA------------------------------Message: 10Date: Mon, 7 Jul 2008 13:20:33 -0600From: "Liz Chlipala" Subject: RE: [Histonet] Antibody for the detection of human cells    needed=0ATo: "Alexandra Meinl" ,    Message-ID:    Content-Type: text/plain;    charset="us-ascii"AlexandraWe have tried the human anti-nuclear antibody from chemicon but we didnot have much success with it.  We have used a human mitochondrialantibody from Novus with good success but in rat tissues rather thanmouse, it's generated in mouse but as long as you use the correctcontrols it should work.  We have also ran In-situ with a repeatsequence for human DNA and that worked nicely between mouse and also ratand human cells.Â=A0 We had our probe made by GeneDetect.Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHCManagerPremier Laboratory, LLCP.O. Box 18592Boulder, CO 80308phone (303) 682-3949fax (303) 682-9060liz@premierlab.comwww.premierlab.comShip to Address:Premier Laboratory, LLC1567 Skyway DriveUnit ELongmont, CO 80504-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf OfAlexandra Meinl=0ASent: Monday, July 07, 2008 12:24 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Antibody for the detection of human cells needed=0AHi all,I'm searching for an antibody for the detection of human cells in mousetissue.And: we don't know what type of cells we're trying to find.I thought about an ab like a-hu-nuclear membrane or GM 130.=0ADoes anybody this? What antibody would you recommend?Any suggestions greatly appreaciated!Alexandra_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu=0Ahttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 11Date: Mon, 7 Jul 2008 15:34:27 -0400From: "Robert Richmond" Subject: [Histonet] Re: H&E quality checkTo: histonet@lists.utsouthwestern.eduMessage-ID:    Content-Type: text/plain; charset=ISO-8859-1Well, I've worked in roughly 60 hospital and private lab pathologyservices in my locum tenens career, and I've never seen a functioningQA (or whatever they call it this year) program for H & E (or anyother stain) in a pathology lab. An occasional lab has the pathologistfill out QA sheets telling them that the slides are wonderful -invariably in such labs the slides are horrible. In most labs nobodybut the pathologist ever looks at a slide under a microscope.A meaningful QA program would have a pathologist and a seniorhistotechnologist review some of the day's production, during theworking day. I've always been ridiculed for suggesting it.Bob RichmondSamurai PathologistKnoxville TN------------------------------Message: 12Date: Mon, 07 Jul 2008 15:47:21 -0400From: "Angela Bitting" Subject: Re: [Histonet] H&E quality checkTo: Cc: histonet ,    histonet-bounces@lists.utsouthwestern.eduMessage-ID: <48723A89.2B7F.00C9.0@geisinger.edu>Content-Type: text/plain; charset=US-ASCIIThanks for all of the replies. I guess I need to rephrase my question. I'm wondering how many slides are reviewed daily under the scope for cutting artifacts, incomplete margins, etc. Where I worked before, we checked 10% of the slides under the scope before sending them on to the Pathologists. Do other labs do this?>>>  7/7/2008 9:49 AM >>>Everywhere I have worked there was a liver control slide run daily for Q.C. The techs checked the staining quality before beginning staining for the day. The slide was dated and documented in the log book.Stephanie D. RiveraSafety Assessment DepartmentGlaxoSmithKline709 Swedeland RDKing of Prussia, PA 19406phone: 610-270-7340fax: 610-270-7202"Angela Bitting"  Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Jul-2008 07:59To"histonet" ccSubject[Histonet] H&E quality check=0AJust curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------Message: 13Date: Mon, 7 Jul 2008 15:50:45 -0400From: "Michael Mihalik" Subject: RE: [Histonet] Re: H&E quality checkTo: "'Robert Richmond'" ,  Â=A0 Message-ID: Content-Type: text/plain;    charset="US-ASCII"To tag along with this statement.  Wouldn't it be nice if the pathologistcould comment on the 'quality' of the stain as they are reviewing the slidein the first place?...or am I missing something here?Michael MihalikPathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540| fax: 270.423.0968-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu=0A[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert=0ARichmondSent: Monday, July 07, 2008 3:34 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Re: H&E quality checkWell, I've worked in roughly 60 hospital and private lab pathologyservices in my locum tenens career, and I've never seen a functioningQA (or whatever they call it this year) program for H & E (or anyother stain) in a pathology lab. An occasional lab has the pathologistfill out QA sheets telling them that the slides are wonderful -invariably in such labs the slides are horrible. In most labs nobodybut the pathologist ever looks at a slide under a microscope.A meaningful QA program would have a pathologist and a seniorhistotechnologist review some of the day's production, during theworking day. I've always been ridiculed for suggesting it.Bob RichmondSamurai PathologistKnoxville TN_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet=0A------------------------------Message: 14Date: Mon, 07 Jul 2008 15:02:20 -0500From: "LINDA MARGRAF" =0ASubject: [Histonet] HT position availableTo: Cc: BETHTEWS@HOTMAIL.COMMessage-ID: <48722FFC020000DA0002B8BF@CNET3.CHILDRENS.COM>Content-Type: text/plain; charset="iso-8859-15"Here's a message Beth asked me to post for her.Please reply to her if you are interested. ThanksLM (histonet administrator)=0ATechnical Applications specialist- laboratoryESSENTIAL DUTIES AND RESPONSIBILITIES Installation of  instrumentation and conducting user training tosuccessfully integrate  products within the customer laboratory.Provide troubleshooting and minor repair to  instrumentation.Work with customers to resolve reagent/staining application issues.Communicate with dispatch and area management on daily/weekly/monthlyactivities (eg. Salesforce.com)Maintaining tools and test equipment in accordance with QualityproceduresConduct in-service training for customers.Customer follow-upSubmit administrative paperwork in a timely and accurate manner.installation formsservice work ordersexpense reportsTravel extensively within the region and throughout the country.REQUIRED EDUCATION & EXPERIENCE Associates degree (or equivalent) or higher in Applied Sciences oraccredited Medical/Histology Technology course.=C2  Minimum one yeargeneral clinical laboratory experience or technical equivalent which mayinclude general histology, cytology, immunohistochemistry, in situhybridization, biology research or work with automated staininginstrumentation. CERTIFICATES, LICENSES, REGISTRATIONS Highly desirable:HISTOLOGY TECHNICIANAmerican Society of Clinical Pathologists Certification HT or HTL.American Society of Clinical Pathologists Qualification inImmunohistochemistry (QIHC).KNOWLEDGE, SKILLS & ABILITIESMust have the ability to read, analyze, and interpret technicalprocedures or governmental regulations (CAP, CLIA).  Ability to writereports. Ability to effectively present information and respond toquestions from customers.  Ability to calculate antibody dilutions.Ability to interpret a variety of instructions furnished in written,verbal, diagram, or schedule form.WORK ENVIRONMENT  The work environment characteristics described here are representativeof those an employee encounters while performing the essential functionsof this job traveling to and working within a Clinical Pathology Lab,Research lab, and animal diagnostic lab. Reasonable accommodations maybe made to enable individuals with disabilities to perform the essentialfunctions.  OTHER QUALIFICATIONSAbility to travel.Valid driver*s license. with a clean driving record.COMPENSATION PACKAGEPOSITION OFFERS A COMPETITVE BASE SALARY AND A CAR PLUS ALL TRAVELEXPENSES AND BENEFITS.CONTACT BETH TEWS RECRUITING BETHTEWS@HOTMAIL.COM623-742-7227OPENINGS CURRENTLY NEED TO BE FILLED BY 715/08 IN PHOENIX, NYC/NJ,STLOUIS, BOSTON MORE TO COME SO PLEASE APPLY