[Histonet] Re: Histonet Digest, Vol 56, Issue 9

From:richard shook



VWR has the metal slide trays  #48469-004----- Original Message ----From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.eduSent: Tuesday, July 8, 2008 8:06:49 AMSubject: Histonet Digest, Vol 56, Issue 9Send Histonet mailing list submissions to    histonet@lists.utsouthwestern.eduTo subscribe or unsubscribe via the World Wide Web, visit    http://lists.utsouthwestern.edu/mailman/listinfo/histonetor, via email, send a message with subject or body 'help' to    histonet-request@lists.utsouthwestern.eduYou can reach the person managing the list at    histonet-owner@lists.utsouthwestern.eduWhen replying, please edit your Subject line so it is more specificthan "Re: Contents of Histonet digest..."Today's Topics:  1. Metal Slide Trays (Metzger, Kenneth)  2. RE: H&E quality check (Christine Bark)  3. RE: Metal Slide Trays (Bartlett, Jeanine (CDC/CCID/NCZVED))  4. Vector Labs Nova HRP--Red Substrate: Not Really EtOH      compatible? (JR R)  5. Help needed for brain processing (Shirley Powell)=0A  6. Antibody for the detection of human cells needed (Alexandra Meinl)  7. tissue processor (Atoska Gentry)  8. Re: Gomeri Iron Stain (Maxim_71@mail.ru)  9. Leitz 1512 rotary microtome (sharon.osborn@comcast.net)  10. RE: Antibody for the detection of human cells needed      (Liz Chlipala)  11. Re: H&E quality check (Robert Richmond)  12. Re: H&E quality check (Angela Bitting)  13. RE: Re: H&E quality check (Michael Mihalik)  14. HT position available (LINDA MARGRAF)  15. RE: H&E quality check (Weems, Joyce)  16. RE: Re: H&E quality check.    . (Henry, Charlene)  17. RE: Re: H&E quality check.    . (Michael Mihalik)  18. RE: Gomeri Iron Stain (Tony Henwood)  19. Re: H&E quality check. (Dave & Lee Mayhew)  20. RE: Re: H&E quality check. (Michael Mihalik)  21. Re: Re: H&E quality check. (Dave & Lee Mayhew)  22. Re: Re: H&E quality check. . (Anne van Binsbergen)=C2 23. Re: H&E quality check (Rene J Buesa)  24. RE: Re: H&E quality check. . (Cheri Miller)----------------------------------------------------------------------Message: 1Date: Mon, 7 Jul 2008 11:33:15 -0600From: "Metzger, Kenneth" Subject: [Histonet] Metal Slide TraysTo: Message-ID:    Content-Type: text/plain;  =A0 charset="iso-8859-1"Does anyone know where I can order the 20 slot metal slide trays? Thanks,kenKen Metzger HTL(ASCP)Histology SupervisorARUP Laboratories500 Chipeta waySalt Lake City, UT 84108801.583.2787 ext 3101- ------------------------------------------------------------------The information transmitted by this e-mail and any includedattachments are from ARUP Laboratories and are intended only for therecipient. The information contained in this message is confidentialand may constitute inside or non-public information underinternational, federal, or state securities laws, or protected healthinformation and is intended only for the use of the recipient.Unauthorized forwarding, printing, copying, distributing, or use ofsuch information is strictly prohibited and may be unlawful. If youare not the intended recipient, please promptly delete this e-mailand notify the sender of the delivery error or you may call ARUPLaboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1(800) 522-2787 ext. 2100------------------------------Message: 2Date: Mon, 7 Jul 2008 10:37:27 -0700From: "Christine Bark" Subject: [Histonet] RE: H&E quality checkTo: histonet@lists.utsouthwestern.eduMessage-ID:=0A    Content-Type: text/plain; charset=us-asciiWe check several different slides from our first rack of the day for H&Eand cutting quality.Christine Bark HT(ASCP)Senior Histotech, PathologySaddleback Memorial Medical Center949-452-3548cbark@memorialcare.org=0A-----Original Message-----From: "Angela Bitting" Subject: [Histonet] H&E quality checkJust curious as to how other hospital labs quality check their H&Eslides? Do most review a percentage microscopically? ______________________________________________________________________________Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.------------------------------Message: 3Date: Mon, 7 Jul 2008 13:40:13 -0400From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Metal Slide TraysTo: "Metzger, Kenneth" ,    histonet@lists.utsouthwestern.edu=0AMessage-ID:    <1CE1847DFEA0A647B1CCDE4108EA60A7F23C7E@LTA3VS011.ees.hhs.gov>Content-Type: text/plain; charset=us-ascii=0AI think this is what you are looking for.  http://www.daigger.com/catalog/product?deptId=&prodId=16050Jeanine BartlettInfectious Diseases Pathology Branch(404) 639-3590 jeanine.bartlett@cdc.hhs.gov-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger,KennethSent: Monday, July 07, 2008 1:33 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Metal Slide Trays=0ADoes anyone know where I can order the 20 slot metal slide trays?Thanks,kenKen Metzger HTL(ASCP)Histology SupervisorARUP Laboratories500 Chipeta waySalt Lake City, UT 84108801.583.2787 ext 3101- ------------------------------------------------------------------The information transmitted by this e-mail and any included attachmentsare from ARUP Laboratories and are intended only for the recipient. Theinformation contained in this message is confidential and may constituteinside or non-public information under international, federal, or state=0Asecurities laws, or protected health information and is intended onlyfor the use of the recipient.Unauthorized forwarding, printing, copying, distributing, or use of suchinformation is strictly prohibited and may be unlawful. If you are notthe intended recipient, please promptly delete this e-mail and notifythe sender of the delivery error or you may call ARUP LaboratoriesCompliance Hot Line in Salt Lake City, Utah USA at (+1=0A(800) 522-2787 ext. 2100_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 4Date: Mon, 7 Jul 2008 10:54:49 -0700From: JR R Subject: [Histonet] Vector Labs Nova HRP--Red Substrate: Not Really    EtOH =A0  compatible?To: Message-ID: Content-Type: text/plain; charset="Windows-1252"The Product information says: "NovaRED™ substrate may bedehydrated in ethanol and cleared in organic solvents such as xyleneand permanently mounted in a non-aqueous mounting medium such asVectaMount"But I have done two stains now where I initially had intense, specific staining at first--But after my slides come out of the EtOH/Xylene for coverslipping, the staining has vanished.=0AThis is getting to be expensive!Has anyone else ever had this problem with Nova Red?  Any ideas?Jerry RicksResearch ScientistUniversity of WashingtonDepartment of Pathology_________________________________________________________________Need to know now? Get instant answers with Windows Live Messenger.http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_messenger_072008------------------------------Message: 5Date: Mon, 07 Jul 2008 14:07:27 -0400From: Shirley Powell Subject: [Histonet] Help needed for brain processingTo: histonet@lists.utsouthwestern.eduMessage-ID: <01MWVKZ3Y6Y200ANAP@Macon2.Mercer.edu>Content-Type: text/plain; charset=us-asciiA friend of mine, who has been banned from histonet by a micro-managingemployer, needs advice from those who use the microwave to expedite fixationof whole or partial brains for routine processing, or any other rapid meansof getting complete fixation on brain. Please send all responses to mepersonally at this address and I will forward the information to them.  Thanks in advance for your help.Shirley Powell------------------------------=0AMessage: 6Date: Mon, 7 Jul 2008 20:24:22 +0200From: "Alexandra Meinl" Subject: [Histonet] Antibody for the detection of human cells neededTo: histonet@lists.utsouthwestern.eduMessage-ID:    Content-Type: text/plain; charset=UTF-8Hi all,=0AI'm searching for an antibody for the detection of human cells in mousetissue.And: we don't know what type of cells we're trying to find.=0AI thought about an ab like a-hu-nuclear membrane or GM 130.Does anybody this? What antibody would you recommend?Any suggestions greatly appreaciated!Alexandra------------------------------Message: 7Date: Mon, 07 Jul 2008 13:35:13 -0500From: Atoska Gentry Subject: [Histonet] tissue processorTo: Histonet Message-ID: <487261E1.3020101@vetmed.auburn.edu>Content-Type: text/plain; charset=ISO-8859-1; format=flowed=0Ahello, please will someone who has info on a modern tissue processor =0Acomparable to an obsolete  25 yr.Autotechnicon Ultra II Tissue Processor please contact me with specifications, product info & pricing ASAP? Thanks, Atoska------------------------------Message: 8Date: Mon, 7 Jul 2008 22:30:20 +0400From: Maxim_71@mail.ruSubject: Re: [Histonet] Gomeri Iron StainTo: histology@gradymem.orgCc: histonet@lists.utsouthwestern.eduMessage-ID: <228377341.20080707223020@mail.ru>Content-Type: text/plain; charset=us-asciiAngie:We never used MWO modification for Gomori Iron Stain.Iron is beautifully can demonsrtate at RT for 10 mins.Vapours of cyanid is very toxic for personnel.=0AMaxim PeshkovRussia,Taganrog.------------------------------Message: 9Date: Mon, 07 Jul 2008 19:11:41 +0000From: sharon.osborn@comcast.netSubject: [Histonet] Leitz 1512 rotary microtomeTo: histonet@lists.utsouthwestern.eduMessage-ID:  =A0 <070720081911.18025.48726A6D0006F323000046692215555884029D010D9C01D202019D0E089C@comcast.net>    Histonetters,  =C2   I have a copy of the instructions manual for above referenced microtome should anyone still have this workhorse and need the instructions.  Please send me your contact information and I am happy to send out.  If there is more than one person requesting it, I can make copies.=0Asharon osbornLab VisionThermo FisherFremont, CA------------------------------Message: 10Date: Mon, 7 Jul 2008 13:20:33 -0600From: "Liz Chlipala" Subject: RE: [Histonet] Antibody for the detection of human cells    needed=0ATo: "Alexandra Meinl" ,    Message-ID:    Content-Type: text/plain;    charset="us-ascii"AlexandraWe have tried the human anti-nuclear antibody from chemicon but we didnot have much success with it.  We have used a human mitochondrialantibody from Novus with good success but in rat tissues rather thanmouse, it's generated in mouse but as long as you use the correctcontrols it should work.  We have also ran In-situ with a repeatsequence for human DNA and that worked nicely between mouse and also ratand human cells.=A0 We had our probe made by GeneDetect.Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHCManagerPremier Laboratory, LLCP.O. Box 18592Boulder, CO 80308phone (303) 682-3949fax (303) 682-9060liz@premierlab.comwww.premierlab.comShip to Address:Premier Laboratory, LLC1567 Skyway DriveUnit ELongmont, CO 80504-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf OfAlexandra Meinl=0ASent: Monday, July 07, 2008 12:24 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Antibody for the detection of human cells needed=0AHi all,I'm searching for an antibody for the detection of human cells in mousetissue.And: we don't know what type of cells we're trying to find.I thought about an ab like a-hu-nuclear membrane or GM 130.=0ADoes anybody this? What antibody would you recommend?Any suggestions greatly appreaciated!Alexandra_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu=0Ahttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 11Date: Mon, 7 Jul 2008 15:34:27 -0400From: "Robert Richmond" Subject: [Histonet] Re: H&E quality checkTo: histonet@lists.utsouthwestern.eduMessage-ID:    Content-Type: text/plain; charset=ISO-8859-1Well, I've worked in roughly 60 hospital and private lab pathologyservices in my locum tenens career, and I've never seen a functioningQA (or whatever they call it this year) program for H & E (or anyother stain) in a pathology lab. An occasional lab has the pathologistfill out QA sheets telling them that the slides are wonderful -invariably in such labs the slides are horrible. In most labs nobodybut the pathologist ever looks at a slide under a microscope.A meaningful QA program would have a pathologist and a seniorhistotechnologist review some of the day's production, during theworking day. I've always been ridiculed for suggesting it.Bob RichmondSamurai PathologistKnoxville TN------------------------------Message: 12Date: Mon, 07 Jul 2008 15:47:21 -0400From: "Angela Bitting" Subject: Re: [Histonet] H&E quality checkTo: Cc: histonet ,    histonet-bounces@lists.utsouthwestern.eduMessage-ID: <48723A89.2B7F.00C9.0@geisinger.edu>Content-Type: text/plain; charset=US-ASCIIThanks for all of the replies. I guess I need to rephrase my question. I'm wondering how many slides are reviewed daily under the scope for cutting artifacts, incomplete margins, etc. Where I worked before, we checked 10% of the slides under the scope before sending them on to the Pathologists. Do other labs do this?>>>  7/7/2008 9:49 AM >>>Everywhere I have worked there was a liver control slide run daily for Q.C. The techs checked the staining quality before beginning staining for the day. The slide was dated and documented in the log book.Stephanie D. RiveraSafety Assessment DepartmentGlaxoSmithKline709 Swedeland RDKing of Prussia, PA 19406phone: 610-270-7340fax: 610-270-7202"Angela Bitting"  Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Jul-2008 07:59To"histonet" ccSubject[Histonet] H&E quality check=0AJust curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------Message: 13Date: Mon, 7 Jul 2008 15:50:45 -0400From: "Michael Mihalik" Subject: RE: [Histonet] Re: H&E quality checkTo: "'Robert Richmond'" ,  =A0 Message-ID: Content-Type: text/plain;    charset="US-ASCII"To tag along with this statement.  Wouldn't it be nice if the pathologistcould comment on the 'quality' of the stain as they are reviewing the slidein the first place?...or am I missing something here?Michael MihalikPathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540| fax: 270.423.0968-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu=0A[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert=0ARichmondSent: Monday, July 07, 2008 3:34 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Re: H&E quality checkWell, I've worked in roughly 60 hospital and private lab pathologyservices in my locum tenens career, and I've never seen a functioningQA (or whatever they call it this year) program for H & E (or anyother stain) in a pathology lab. An occasional lab has the pathologistfill out QA sheets telling them that the slides are wonderful -invariably in such labs the slides are horrible. In most labs nobodybut the pathologist ever looks at a slide under a microscope.A meaningful QA program would have a pathologist and a seniorhistotechnologist review some of the day's production, during theworking day. I've always been ridiculed for suggesting it.Bob RichmondSamurai PathologistKnoxville TN_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet=0A------------------------------Message: 14Date: Mon, 07 Jul 2008 15:02:20 -0500From: "LINDA MARGRAF" =0ASubject: [Histonet] HT position availableTo: Cc: BETHTEWS@HOTMAIL.COMMessage-ID: <48722FFC020000DA0002B8BF@CNET3.CHILDRENS.COM>Content-Type: text/plain; charset="iso-8859-15"Here's a message Beth asked me to post for her.Please reply to her if you are interested. ThanksLM (histonet administrator)=0ATechnical Applications specialist- laboratoryESSENTIAL DUTIES AND RESPONSIBILITIES Installation of  instrumentation and conducting user training tosuccessfully integrate  products within the customer laboratory.Provide troubleshooting and minor repair to  instrumentation.Work with customers to resolve reagent/staining application issues.Communicate with dispatch and area management on daily/weekly/monthlyactivities (eg. Salesforce.com)Maintaining tools and test equipment in accordance with QualityproceduresConduct in-service training for customers.Customer follow-upSubmit administrative paperwork in a timely and accurate manner.installation formsservice work ordersexpense reportsTravel extensively within the region and throughout the country.REQUIRED EDUCATION & EXPERIENCE Associates degree (or equivalent) or higher in Applied Sciences oraccredited Medical/Histology Technology course.=C2 Minimum one yeargeneral clinical laboratory experience or technical equivalent which mayinclude general histology, cytology, immunohistochemistry, in situhybridization, biology research or work with automated staininginstrumentation. CERTIFICATES, LICENSES, REGISTRATIONS Highly desirable:HISTOLOGY TECHNICIANAmerican Society of Clinical Pathologists Certification HT or HTL.American Society of Clinical Pathologists Qualification inImmunohistochemistry (QIHC).KNOWLEDGE, SKILLS & ABILITIESMust have the ability to read, analyze, and interpret technicalprocedures or governmental regulations (CAP, CLIA).  Ability to writereports. Ability to effectively present information and respond toquestions from customers.  Ability to calculate antibody dilutions.Ability to interpret a variety of instructions furnished in written,verbal, diagram, or schedule form.WORK ENVIRONMENT  The work environment characteristics described here are representativeof those an employee encounters while performing the essential functionsof this job traveling to and working within a Clinical Pathology Lab,Research lab, and animal diagnostic lab. Reasonable accommodations maybe made to enable individuals with disabilities to perform the essentialfunctions.  OTHER QUALIFICATIONSAbility to travel.Valid driver*s license. with a clean driving record.COMPENSATION PACKAGEPOSITION OFFERS A COMPETITVE BASE SALARY AND A CAR PLUS ALL TRAVELEXPENSES AND BENEFITS.CONTACT BETH TEWS RECRUITING BETHTEWS@HOTMAIL.COM623-742-7227OPENINGS CURRENTLY NEED TO BE FILLED BY 715/08 IN PHOENIX, NYC/NJ,STLOUIS, BOSTON MORE TO COME SO PLEASE APPLY
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------------------------------Message: 15Date: Mon, 7 Jul 2008 16:14:06 -0400From: "Weems, Joyce" Subject: RE: [Histonet] H&E quality checkTo: "Angela Bitting" ,  =A0 Cc: histonet ,    histonet-bounces@lists.utsouthwestern.eduMessage-ID:    <982A0A9461F9BF438C7B19A6E425A383373163@ITSSSXM01V6.one.ads.che.org>Content-Type: text/plain; charset="us-ascii"=0AWe do 1 or 2 per full tray - recording wrinkles, folds, poor staining,etc. to get an approximate 10% reviewed. -----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AngelaBittingSent: Monday, July 07, 2008 3:47 PMTo: stephanie.d.rivera@gsk.comCc: histonet; histonet-bounces@lists.utsouthwestern.eduSubject: Re: [Histonet] H&E quality checkThanks for all of the replies. I guess I need to rephrase my question.I'm wondering how many slides are reviewed daily under the scope forcutting artifacts, incomplete margins, etc. Where I worked before, wechecked 10% of the slides under the scope before sending them on to thePathologists. Do other labs do this?>>> 7/7/2008 9:49 AM >>>Everywhere I have worked there was a liver control slide run daily forQ.C. The techs checked the staining quality before beginning stainingfor the day. The slide was dated and documented in the log book.Stephanie D. RiveraSafety Assessment DepartmentGlaxoSmithKline709 Swedeland RDKing of Prussia, PA 19406phone: 610-270-7340fax: 610-270-7202"Angela Bitting" Sent by:histonet-bounces@lists.utsouthwestern.edu07-Jul-2008 07:59To"histonet" ccSubject[Histonet] H&E quality checkJust curious as to how other hospital labs quality check their H&Eslides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documentsattached to it, if any) is confidential and may be legally privileged.It is intended solely for the addressee. Access to this message byanyone else is unauthorized. If you are not the intended recipient, anydisclosure, copying, distribution or any action taken, or omitted to betaken, in reliance on it is prohibited and may be unlawful. If you havereceived this message in error, please delete all electronic copies ofthis message (and the documents attached to it, if any), destroy anyhard copies you may have created and notify me immediately by replyingto this email. Thank you._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet =0A_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetConfidentiality Notice:This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).  It may contain information that is privileged and confidential.  Any unauthorized review, use,disclosure, or distribution is prohibited. If you are =0Anot the intended recipient, please reply to the sender that you have received the message in error, then delete this message.------------------------------Message: 16Date: Mon, 7 Jul 2008 15:14:08 -0500From: "Henry, Charlene" Subject: RE: [Histonet] Re: H&E quality check.    .To: 'Michael Mihalik' , 'Robert Richmond'    , "histonet@lists.utsouthwestern.edu"    Message-ID:    <03E1F5968F60C5448635D49D38B283ED01460FD918@SJMEMXMBS11.stjude.sjcrh.local> =A0  Content-Type: text/plain; charset="us-ascii"Michael,=0AActually it is now a CAP requirement.ANP.11713 Is there documented evidence of daily review of the technical quality of histologic preparations by the pathologist.This is the way we address this question.Each morning while we are cutting surgical and bone marrow biopsies, we randomly select 2-3 blocks and cut a QC H&E slide in addition to the routine cuts. They are stained along the routine cases and these are given to our Director of AP for review. She then documents the quality of fixation, processing, cutting and staining on a log. While she is on vacation one of our other pathologists review the slides. When the log sheet is full, it is returned to me and filed with our QC records.Charlene Henry HT (ASCP), QIHCAnatomic Pathology Section HeadDepartment of PathologySt. Jude Children's Research Hospital901-495-3191fax 901-495-3100-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael MihalikSent: Monday, July 07, 2008 2:51 PMTo: 'Robert Richmond'; histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] Re: H&E quality check. .=0ATo tag along with this statement.  Wouldn't it be nice if the pathologist could comment on the 'quality' of the stain as they are reviewing the slide in the first place?...or am I missing something here?Michael MihalikPathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540| fax: 270.423.0968-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert RichmondSent: Monday, July 07, 2008 3:34 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Re: H&E quality checkWell, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope.A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it.Bob RichmondSamurai PathologistKnoxville TN_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 17Date: Mon, 7 Jul 2008 16:47:31 -0400From: "Michael Mihalik" Subject: RE: [Histonet] Re: H&E quality check.    .To: "'Henry, Charlene'" ,    "'Robert    Richmond'" ,    Message-ID: Content-Type: text/plain;    charset="US-ASCII"I appreciate the response.  Let me ask this question another way.  Is there=0Aanybody who currently logs their histo QC in their computer system, not onpaper, not on some department created excel spreadsheet?Would this not be desirable?Michael MihalikPathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540| fax: 270.423.0968=0A-----Original Message-----From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, July 07, 2008 4:14 PMTo: 'Michael Mihalik'; 'Robert Richmond'; histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] Re: H&E quality check. .Michael,Actually it is now a CAP requirement.ANP.11713 Is there documented evidence of daily review of the technicalquality of histologic preparations by the pathologist.=0AThis is the way we address this question.Each morning while we are cutting surgical and bone marrow biopsies, werandomly select 2-3 blocks and cut a QC H&E slide in addition to the routinecuts. They are stained along the routine cases and these are given to ourDirector of AP for review. She then documents the quality of fixation,processing, cutting and staining on a log. While she is on vacation one ofour other pathologists review the slides. When the log sheet is full, it isreturned to me and filed with our QC records.Charlene Henry HT (ASCP), QIHCAnatomic Pathology Section HeadDepartment of PathologySt. Jude Children's Research Hospital901-495-3191fax 901-495-3100-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MichaelMihalikSent: Monday, July 07, 2008 2:51 PMTo: 'Robert Richmond'; histonet@lists.utsouthwestern.eduSubject: RE: [Histonet] Re: H&E quality check. .To tag along with this statement.  Wouldn't it be nice if the pathologist=0Acould comment on the 'quality' of the stain as they are reviewing the slidein the first place?...or am I missing something here?Michael MihalikPathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540| fax: 270.423.0968-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RobertRichmondSent: Monday, July 07, 2008 3:34 PMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Re: H&E quality checkWell, I've worked in roughly 60 hospital and private lab pathology servicesin my locum tenens career, and I've never seen a functioning QA (or whateverthey call it this year) program for H & E (or any other stain) in apathology lab. An occasional lab has the pathologist fill out QA sheetstelling them that the slides are wonderful - invariably in such labs theslides are horrible. In most labs nobody but the pathologist ever looks at aslide under a microscope.A meaningful QA program would have a pathologist and a seniorhistotechnologist review some of the day's production, during the workingday. I've always been ridiculed for suggesting it.Bob RichmondSamurai PathologistKnoxville TN_______________________________________________=0AHistonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet=0A------------------------------Message: 18Date: Tue, 8 Jul 2008 09:19:30 +1000From: "Tony Henwood" =0ASubject: RE: [Histonet] Gomeri Iron StainTo: "Histology Dept" ,    =0AMessage-ID: =0AContent-Type: text/plain; charset="us-ascii"The following works well though it is a Perl's stain. I can't rememberthe Gomori Iron stain. The old cranium is shrinking as I get older!!It was developed for frozen sections (eg differentiating melanin fromiron pigment) but works quite well for paraffin sections.1    Placed ethanol fixed frozen sections or hydrated paraffinsection in an the Perl's reagent in a plastic coplin jar (20ml each of2% Potassium ferrocyanide and 2% HCl).Microwave for 20 sec at 650watts.2.    Leave sections in the hot solution for up to 3 minutes, removeif the solution becomes cloudy.3.    Rinse in water.4.    Counterstain with eosin (as used for the frozen section H&E) ora red nuclear stain (Nuclear Fast Red or Neutral red).5.    Rinse in water, DC&M.Reference:    Henwood (2002) "Microwave Perl's Stain for Urgent FrozenSections" Aust J Med Sc 23(2):68-69.RegardsTony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)Laboratory Manager & Senior ScientistThe Children's Hospital at Westmead,Locked Bag 4001, Westmead, 2145, AUSTRALIA.Tel: 612 9845 3306Fax: 612 9845 3318-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf OfHistology DeptSent: Tuesday, 8 July 2008 1:50 AMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Gomeri Iron Stain=0AWe finally got a microwave for special stains.  Can you use a microwavemethod to do Gomeri Iron Stain?  If so, would someone please share youprocedure with us?Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet*********************************************************************This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at WestmeadThis note also confirms that this email message has beenvirus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.**********************************************************************------------------------------Message: 19Date: Mon, 7 Jul 2008 20:45:29 -0400From: "Dave & Lee Mayhew" Subject: [Histonet] Re: H&E quality check.To: "Histonet" Message-ID: <008601c8e093$eb805690$e013c143@user4ab93baede>Content-Type: text/plain;    charset=3D"iso-8859-1"Hi Michael,We use Meditech and create a daily QC specimen.  In the data fields for the QC specimen we record all the stains we did for that day, including H&E, and all the specimen numbers that were controlled by those QC slides.  Any problems, concerns, repeats, etc. are recorded in the specimen comment field.A daily report is printed out at the end of the day and filed in the QC binder.Lee Mayhew MLTSt. Joseph's HospitalHamilton  ON  Canada=0A------------------------------Message: 20Date: Mon, 7 Jul 2008 21:16:13 -0400From: "Michael Mihalik" Subject: RE: [Histonet] Re: H&E quality check.To: "'Dave & Lee Mayhew'" ,    "'Histonet'"    Message-ID: <772604D2DC974271BCC24D75ED3FA5ED@MDMM1330>Content-Type: text/plain;    charset="US-ASCII"=0ASo you create a 'normal' case where the specimen is a qc case and then fillin all the 'normal' fields with qc data.Is that correct?....or are there specialized qc data fields, like a prompt for stainquality, block quality, etc.I'm looking to see if any computer system does anything special for QCpurposes, relative to block, slides, stains, etc.Michael MihalikPathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540| fax: 270.423.0968-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dave & LeeMayhew=0ASent: Monday, July 07, 2008 8:45 PMTo: HistonetSubject: [Histonet] Re: H&E quality check.Hi Michael,We use Meditech and create a daily QC specimen.  In the data fields for the QC specimen we record all the stains we did forthat day, including H&E, and all the specimen numbers that were controlledby those QC slides.  Any problems, concerns, repeats, etc. are recorded inthe specimen comment field.A daily report is printed out at the end of the day and filed in the QCbinder.Lee Mayhew MLTSt. Joseph's HospitalHamilton  ON=C2 Canada_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 21Date: Mon, 7 Jul 2008 21:34:08 -0400From: "Dave & Lee Mayhew" Subject: Re: [Histonet] Re: H&E quality check.To: "Michael Mihalik" ,    "'Histonet'"    Message-ID: <00a501c8e09a$b7876bb0$e013c143@user4ab93baede>Content-Type: text/plain; format=flowed; charset="iso-8859-1";    reply-type=originalMichaelThe case created is a special QC Specimen.=C2 Meditech allows you to create any data fields that you want, they do not have to be the same data sections as are found in patient specimens, so you could create a prompt for the things you suggest if you wanted to.  The workload that you enter into this specimen is counted as quality control not patient workload.I should say that we also have a daily sheet that the day's pathologist fills out to document any of their concerns.  We do not enter this into Meditech, although we, or they, could.  Lee Mayhew MLT> St. Joseph's Hospital> Hamilton  ON  Canada------------------------------Message: 22Date: Tue, 8 Jul 2008 08:52:10 +0400From: "Anne van Binsbergen" Subject: Re: [Histonet] Re: H&E quality check. .To: "Michael Mihalik" Cc: histonet@lists.utsouthwestern.edu, "Henry,    Charlene" =C2  , Robert Richmond Message-ID:    Content-Type: text/plain; charset=ISO-8859-1=0Awe have a tissue array (tonsil, kidney, skin, L/N, appendix) block which wemade ourselves by using a dermatology punch toolidentical slides are cut and kept in a boxeach morning we stain one and it gets viewed by a senior tech - the pathdoes not have time for such matters!!results logged, adjustments noted, signed off, logs filed, slides filed -all ready for CAP to scrutiniseif we adjust the stain (or anything else) we run another QC slide and relogthe resultswe are in the process of making an extended array for a whole bunch ofspecials tooits a pain in the @$$ but my staff are used to the process nowideally it should be done the 'samurai' way - but im just the tech - what doi know!!! ;))annieinarabia2008/7/8 Michael Mihalik :> I appreciate the response.  Let me ask this question another way.=A0 Is there> anybody who currently logs their histo QC in their computer system, not on> paper, not on some department created excel spreadsheet?>> Would this not be desirable?>>> Michael Mihalik> PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540> | fax: 270.423.0968>>>>> -----Original Message----->=A0 From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG]> Sent: Monday, July 07, 2008 4:14 PM> To: 'Michael Mihalik'; 'Robert Richmond';> histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Re: H&E quality check. .>> Michael,> Actually it is now a CAP requirement.>=0A> ANP.11713 Is there documented evidence of daily review of the technical> quality of histologic preparations by the pathologist.>> This is the way we address this question.> Each morning while we are cutting surgical and bone marrow biopsies, we> randomly select 2-3 blocks and cut a QC H&E slide in addition to the> routine> cuts. They are stained along the routine cases and these are given to our> Director of AP for review. She then documents the quality of fixation,> processing, cutting and staining on a log. While she is on vacation one of> our other pathologists review the slides. When the log sheet is full, it is> returned to me and filed with our QC records.>>> Charlene Henry HT (ASCP), QIHC> Anatomic Pathology Section Head> Department of Pathology> St. Jude Children's Research Hospital> 901-495-3191> fax 901-495-3100>=0A> -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael> Mihalik> Sent: Monday, July 07, 2008 2:51 PM> To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Re: H&E quality check. .>> To tag along with this statement.  Wouldn't it be nice if the pathologist> could comment on the 'quality' of the stain as they are reviewing the slide> in the first place?>> ...or am I missing something here?>> Michael Mihalik> PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540> | fax: 270.423.0968>>>>> -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert> Richmond> Sent: Monday, July 07, 2008 3:34 PM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Re: H&E quality check>> Well, I've worked in roughly 60 hospital and private lab pathology services> in my locum tenens career, and I've never seen a functioning QA (or> whatever> they call it this year) program for H & E (or any other stain) in a> pathology lab. An occasional lab has the pathologist fill out QA sheets> telling them that the slides are wonderful - invariably in such labs the> slides are horrible. In most labs nobody but the pathologist ever looks at> a> slide under a microscope.>> A meaningful QA program would have a pathologist and a senior> histotechnologist review some of the day's production, during the working> day. I've always been ridiculed for suggesting it.>> Bob Richmond> Samurai Pathologist> Knoxville TN>> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>>> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>>>>> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>-- Anne van Binsbergen (Hope)Abu Dhabi=0AUAE------------------------------Message: 23Date: Tue, 8 Jul 2008 04:46:40 -0700 (PDT)From: Rene J Buesa =0ASubject: Re: [Histonet] H&E quality checkTo: stephanie.d.rivera@gsk.com, Angela Bitting    Cc: histonet ,    histonet-bounces@lists.utsouthwestern.eduMessage-ID: <661700.14357.qm@web65702.mail.ac4.yahoo.com>Content-Type: text/plain; charset=iso-8859-1If you get to a percentage, then you will waste your whole day doing that.What I used to do is to let the pathologists reject the slides, then I reviewed them, found out who cut them, discuss the issue, fill the QA and retrain the HT who did the bad slide.Any other approach will be a total loss of precious time.Don't even think if giving a form to the pathologists to fill out the problem they found, they are not going to do it, and if they do, they will be wasting their time. That is the supervisor's task, the PT task is to reject, the supervisor's task is to prevent the problem from occurring again.René J.--- On Mon, 7/7/08, Angela Bitting wrote:       ------------------------------Message: 24Date: Tue, 8 Jul 2008 07:05:20 -0500From: "Cheri Miller" Subject: RE: [Histonet] Re: H&E quality check. .To: "'Anne van Binsbergen'" ,=C2   "'Michael Mihalik'"    Cc: histonet@lists.utsouthwestern.edu, "'Henry,    Charlene'"    ,  =A0 'Robert Richmond' Message-ID: <000901c8e0f2$e4e24280$3d02a8c0@plab.local>Content-Type: text/plain;    charset="us-ascii"We check the slide quality daily by using a control slide (usually anappendix or gallbladder) with the first rack of slides. I then check randomslides from each rack stained, we also have a QA sheet the paths fill outdaily. They grade us on grossing, specimen sampling, processing, embedding,microtomy, staining and cover slipping. Most of them see this as a truequality control indicator and let us know when we have an issue; a few justfill it out without any real critiquing, which defeats the whole purpose.  Cheryl Miller HT (ASCP)Histology SupervisorPhysicians Laboratory,P.C.Omaha, Ne. 402 738 5052=0APRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.  Any dissemination, distribution, or copying of this e-mail is strictly prohibited.  If you have received this message in error, please notify the sender immediately and delete this email from your system.------------------------------_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetEnd of Histonet Digest, Vol 56, Issue 9*************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/hist
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