at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others.
Jean-Martin Lapointe, DMV, MS, dACVP
1635 Lionel-Bertrand, Boisbriand
Québec, Canada J7H 1N8
tel: 450-435-9482 ext.247
Date: Thu, 26 Jun 2008 09:27:58 -0400
From: "Connolly, Brett M"
Subject: [Histonet] IHC marker for M-phase cells
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I am looking for an antibody for labeling FFPE sections of mitotic cells
that are only in M-phase. Found one article in J Histo Cyto using clone
HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the
Authors produced their own antibody.
The commercial suppliers I have been able to locate do not indicate IHC
as a suitable application for their products.
Anyone had experience with this??
Or can you recommend another M-phase specific antibody?
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
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