Hi histonetters,
Anyone out there successfully using a monoclonal HMB45 antibody on
canine tissues? I think I've read a dozen papers all with different
protocols and my eyes are about to pop out of my head! Currently I'm at
a 1:50 dilution with a pretreatment in a citrate buffer (pressure
cooker). I have my choice of a polymer based detection as well as a
biotinylated detection. Any help would be appreciated. Thanks in
advance.
MaryAnn Dixon BS
Biological Scientist
Anatomic Pathology
UF Veterinary Medical Center
(352) 392-2235 Ext. 4517
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