SigmaAldrich has a large variety of anti-FLAG antibodies. Have the usual mouse culprits but if that is not what you want, I've used their rabbit monoclonal anti-FLAG Ab successfully on mouse tissue (didn't try brain). They also have rabbit polyclonal anti-FLAG but did not try. I'm not a big mouse-on-mouse fan at all. But I have used their directly conjugated mouse monoclonal anti-FLAGs on mouse tissue (again, not brain). Remember, if you don't know, that anti-FLAG antibodies are a bit idiosyncratic. Some MUST have calcium (Ca2+) ions present during incubation or you will get no binding. Some are not like that but many are.
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> I was hoping someone could suggest a good anti-flag antibody for use in mouse
> brain. A lot of anti-flags are monoclonal, which is not very good for mouse
> tissue obviously. Does anyone have a suggestion? The amino acid sequence of
> the flag is DYKDDDDK. A FITC tag would be helpful as well, but right now just
> having a non-mouse antibody would be great. Also, has anyone tried the
> mouse-on-mouse kits in the brain or have suggestions for going this route?
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