From:"Tony Henwood"

This is our protocol:


1.	Fixative (Formal Alcohol)
40% Formaldehyde		200ml
95% Ethanol			1800ml
Add 3 drops of 1% eosin to colour the solution

Warning Toxic - see MSDS

2.	Formic Acid/Formalin Decalcification Solution
Pathtech Cat No ADF.J
(4% Formaldehyde, 33% Formic acid, 0.85% Sodium Chloride)


1.	Allow trephine to fix in formal alcohol for 3  to 4 hours (best overnight)
2.	Rinse the trephine in water and place in Decalcifying solution for 3-4 hours.
3.	Place trephinein biopsy cassette.
4.	Place in 10% formalin to await processing.
5.	If calcium is present when cutting, then surface decal in RDO for 20mion, wash and recut

1.	Blake, J (1970) "Histological Methods of the University of Melbourne" p15.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: [] On Behalf Of MaryAnn Hancock
Sent: Thursday, 21 June 2007 1:53 AM
Subject: [Histonet] BONE MARROW DECAL

I am interested to know what Decal fluid is being used for Bone Marrow Core Biopsies and how long you fix them for.

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