Geoff- would you be willing to post information to Histonet regarding xTH
antibody? Especially order info? Thanks, andra
On Fri, 15 Jun 2007, Geoff McAuliffe wrote:
> Hi Natalie:
>
> I use anti-TH from Pel-Freez in Rogers, Arkansas along with a Vector ABC
> Elite kit. It is relatively easy to get good TH staining in CNS or adrenal
> medulla.
> Are you using fixed, frozen sections or wax embedded?
> Is your peroxide fresh (< 1 year old)?
> How long is the tissue fixed and in what fixative?
>
> Geoff
>
> Natalie Nagorsky wrote:
>> Hi Histonet,
>>
>>
>> I have several protocols for Tirosine Hydroxylase and I was not successful
>> to get good results.
>>
>> May some one share the working protocol and sours for TH.
>>
>>
>> Any help will be appreciate.
>>
>> Natalie
>>
>> Brain Research Center
>>
>> Bar-Ilan University
>>
>> Israel
>>
>> _______________________________________________
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>>
>>
>>
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583 mcauliff@umdnj.edu
> **********************************************
>
>
>
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